BRENDA - Enzyme Database

Biochemical characterization of the reverse activity of rat brain ceramidase. A CoA-independent and fumonisin B1-insensitive ceramide synthase

El Bawab, S.; Birbes, H.; Roddy, P.; Szulc, Z.M.; Bielawska, A.; Hannun, Y.A.; J. Biol. Chem. 276, 16758-16766 (2001)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.23
cardiolipin
activates the ceramidase activity by 2.5fold at 8-10 mol%, inhibits the ceramide synthesis activity, mechanism
Rattus norvegicus
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.23
2-mercaptoethanol
inhibits both ceramidase and ceramide synthesis activity
Rattus norvegicus
3.5.1.23
C16-ceramide
ceramide synthesis activity, 10 mol%, 50% inhibition
Rattus norvegicus
3.5.1.23
cardiolipin
inhibits the ceramide synthesis activity: total inhibition at 2.5-5 mol%, activates the ceramidase activity, mechanism
Rattus norvegicus
3.5.1.23
CuCl2
ceramide synthesis activity, 1 mM, total inhibition
Rattus norvegicus
3.5.1.23
dithiothreitol
inhibits both ceramidase and ceramide synthesis activity
Rattus norvegicus
3.5.1.23
L-erythro-sphingosine
competitive inhibitor of ceramide synthesis activity
Rattus norvegicus
3.5.1.23
lysophosphatidylcholine
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
additional information
the ceramide synthesis activity is not inhibited in vitro and in cells by fuminosin B1, not inhibited by EDTA or ATP, both up to 10 mM
Rattus norvegicus
3.5.1.23
myristaldehyde
competitively inhibits the ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition
Rattus norvegicus
3.5.1.23
palmitaldehyde
ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition
Rattus norvegicus
3.5.1.23
phosphatidic acid
ceramide synthesis activity, 2.5-5 mol%, total inhibition
Rattus norvegicus
3.5.1.23
phosphatidylcholine
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
phosphatidylglycerol
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
phosphatidylserine
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
sphingomyelin
ceramide synthesis activity, 5 mol%, 50% inhibition
Rattus norvegicus
3.5.1.23
ZnCl2
ceramide synthesis activity, 1 mM, total inhibition
Rattus norvegicus
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.23
additional information
-
additional information
kinetic data for the ceramide synthesis reaction, random sequential kinetic mechanism
Rattus norvegicus
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.23
membrane
membrane-bound, brain
Rattus norvegicus
16020
-
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.23
additional information
the ceramide synthesis activity is totally independent of stimulating cations
Rattus norvegicus
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.23
Rattus norvegicus
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.5.1.23
-
Rattus norvegicus
Reaction
EC Number
Reaction
Commentary
Organism
3.5.1.23
a ceramide + H2O = a carboxylate + sphingosine
kinetic mechanism
Rattus norvegicus
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.23
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.23
D-erythro-dihydrosphingosine + fatty acid
very low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
D-erythro-sphingosine + fatty acid
highest ceramide synthesis activity with D-erythro-sphingosine
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
D-threo-sphingosine + fatty acid
low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
L-erythro-sphingosine + fatty acid
low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
L-threo-sphingosine + fatty acid
low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
additional information
no substrate in the ceramide synthesis reaction: palmitoyl-CoA
655998
Rattus norvegicus
?
-
-
-
-
3.5.1.23
myristic acid + sphingosine
highest ceramide synthesis activity with myristic acid
655998
Rattus norvegicus
N-myristoylsphingosine + H2O
-
-
-
?
3.5.1.23
N-acylsphingosine + H2O
CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis
655998
Rattus norvegicus
a carboxylate + sphingosine
-
-
-
r
3.5.1.23
N-palmitoylsphingosine + H2O
-
655998
Rattus norvegicus
palmitic acid + sphingosine
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.23
6.5
7
ceramide synthesis activity, narrow optimum in the neutral range
Rattus norvegicus
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.23
5
8
very low ceramide synthesis activity below pH 5 or above pH 8
Rattus norvegicus
3.5.1.23
5.5
10
broad pH-range, ceramidase activity
Rattus norvegicus
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
3.5.1.23
additional information
coenzyme A-independent mechanism
Rattus norvegicus
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
3.5.1.23
cardiolipin
activates the ceramidase activity by 2.5fold at 8-10 mol%, inhibits the ceramide synthesis activity, mechanism
Rattus norvegicus
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
3.5.1.23
additional information
coenzyme A-independent mechanism
Rattus norvegicus
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.5.1.23
2-mercaptoethanol
inhibits both ceramidase and ceramide synthesis activity
Rattus norvegicus
3.5.1.23
C16-ceramide
ceramide synthesis activity, 10 mol%, 50% inhibition
Rattus norvegicus
3.5.1.23
cardiolipin
inhibits the ceramide synthesis activity: total inhibition at 2.5-5 mol%, activates the ceramidase activity, mechanism
Rattus norvegicus
3.5.1.23
CuCl2
ceramide synthesis activity, 1 mM, total inhibition
Rattus norvegicus
3.5.1.23
dithiothreitol
inhibits both ceramidase and ceramide synthesis activity
Rattus norvegicus
3.5.1.23
L-erythro-sphingosine
competitive inhibitor of ceramide synthesis activity
Rattus norvegicus
3.5.1.23
lysophosphatidylcholine
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
additional information
the ceramide synthesis activity is not inhibited in vitro and in cells by fuminosin B1, not inhibited by EDTA or ATP, both up to 10 mM
Rattus norvegicus
3.5.1.23
myristaldehyde
competitively inhibits the ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition
Rattus norvegicus
3.5.1.23
palmitaldehyde
ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition
Rattus norvegicus
3.5.1.23
phosphatidic acid
ceramide synthesis activity, 2.5-5 mol%, total inhibition
Rattus norvegicus
3.5.1.23
phosphatidylcholine
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
phosphatidylglycerol
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
phosphatidylserine
ceramide synthesis activity, 10 mol%, moderate inhibition
Rattus norvegicus
3.5.1.23
sphingomyelin
ceramide synthesis activity, 5 mol%, 50% inhibition
Rattus norvegicus
3.5.1.23
ZnCl2
ceramide synthesis activity, 1 mM, total inhibition
Rattus norvegicus
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.23
additional information
-
additional information
kinetic data for the ceramide synthesis reaction, random sequential kinetic mechanism
Rattus norvegicus
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
3.5.1.23
membrane
membrane-bound, brain
Rattus norvegicus
16020
-
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
3.5.1.23
additional information
the ceramide synthesis activity is totally independent of stimulating cations
Rattus norvegicus
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.5.1.23
-
Rattus norvegicus
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
3.5.1.23
brain
-
Rattus norvegicus
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.23
D-erythro-dihydrosphingosine + fatty acid
very low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
D-erythro-sphingosine + fatty acid
highest ceramide synthesis activity with D-erythro-sphingosine
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
D-threo-sphingosine + fatty acid
low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
L-erythro-sphingosine + fatty acid
low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
L-threo-sphingosine + fatty acid
low ceramide synthesis activity
655998
Rattus norvegicus
?
-
-
-
?
3.5.1.23
additional information
no substrate in the ceramide synthesis reaction: palmitoyl-CoA
655998
Rattus norvegicus
?
-
-
-
-
3.5.1.23
myristic acid + sphingosine
highest ceramide synthesis activity with myristic acid
655998
Rattus norvegicus
N-myristoylsphingosine + H2O
-
-
-
?
3.5.1.23
N-acylsphingosine + H2O
CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis
655998
Rattus norvegicus
a carboxylate + sphingosine
-
-
-
r
3.5.1.23
N-palmitoylsphingosine + H2O
-
655998
Rattus norvegicus
palmitic acid + sphingosine
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
3.5.1.23
6.5
7
ceramide synthesis activity, narrow optimum in the neutral range
Rattus norvegicus
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
3.5.1.23
5
8
very low ceramide synthesis activity below pH 5 or above pH 8
Rattus norvegicus
3.5.1.23
5.5
10
broad pH-range, ceramidase activity
Rattus norvegicus