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Literature summary extracted from

  • El Bawab, S.; Birbes, H.; Roddy, P.; Szulc, Z.M.; Bielawska, A.; Hannun, Y.A.
    Biochemical characterization of the reverse activity of rat brain ceramidase. A CoA-independent and fumonisin B1-insensitive ceramide synthase (2001), J. Biol. Chem., 276, 16758-16766.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.5.1.23 cardiolipin activates the ceramidase activity by 2.5fold at 8-10 mol%, inhibits the ceramide synthesis activity, mechanism Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.23 2-mercaptoethanol inhibits both ceramidase and ceramide synthesis activity Rattus norvegicus
3.5.1.23 C16-ceramide ceramide synthesis activity, 10 mol%, 50% inhibition Rattus norvegicus
3.5.1.23 cardiolipin inhibits the ceramide synthesis activity: total inhibition at 2.5-5 mol%, activates the ceramidase activity, mechanism Rattus norvegicus
3.5.1.23 CuCl2 ceramide synthesis activity, 1 mM, total inhibition Rattus norvegicus
3.5.1.23 dithiothreitol inhibits both ceramidase and ceramide synthesis activity Rattus norvegicus
3.5.1.23 L-erythro-sphingosine competitive inhibitor of ceramide synthesis activity Rattus norvegicus
3.5.1.23 lysophosphatidylcholine ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
3.5.1.23 additional information the ceramide synthesis activity is not inhibited in vitro and in cells by fuminosin B1, not inhibited by EDTA or ATP, both up to 10 mM Rattus norvegicus
3.5.1.23 myristaldehyde competitively inhibits the ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition Rattus norvegicus
3.5.1.23 palmitaldehyde ceramide synthesis activity, 2.5-3 mol%, about 50% inhibition Rattus norvegicus
3.5.1.23 phosphatidic acid ceramide synthesis activity, 2.5-5 mol%, total inhibition Rattus norvegicus
3.5.1.23 phosphatidylcholine ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
3.5.1.23 phosphatidylglycerol ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
3.5.1.23 phosphatidylserine ceramide synthesis activity, 10 mol%, moderate inhibition Rattus norvegicus
3.5.1.23 sphingomyelin ceramide synthesis activity, 5 mol%, 50% inhibition Rattus norvegicus
3.5.1.23 ZnCl2 ceramide synthesis activity, 1 mM, total inhibition Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.23 additional information
-
additional information kinetic data for the ceramide synthesis reaction, random sequential kinetic mechanism Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.5.1.23 membrane membrane-bound, brain Rattus norvegicus 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.23 additional information the ceramide synthesis activity is totally independent of stimulating cations Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.23 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.23
-
Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.5.1.23 a ceramide + H2O = a carboxylate + sphingosine kinetic mechanism Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.5.1.23 brain
-
Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.23 D-erythro-dihydrosphingosine + fatty acid very low ceramide synthesis activity Rattus norvegicus ?
-
?
3.5.1.23 D-erythro-sphingosine + fatty acid highest ceramide synthesis activity with D-erythro-sphingosine Rattus norvegicus ?
-
?
3.5.1.23 D-threo-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ?
-
?
3.5.1.23 L-erythro-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ?
-
?
3.5.1.23 L-threo-sphingosine + fatty acid low ceramide synthesis activity Rattus norvegicus ?
-
?
3.5.1.23 additional information no substrate in the ceramide synthesis reaction: palmitoyl-CoA Rattus norvegicus ?
-
?
3.5.1.23 myristic acid + sphingosine highest ceramide synthesis activity with myristic acid Rattus norvegicus N-myristoylsphingosine + H2O
-
?
3.5.1.23 N-acylsphingosine + H2O CDase cleaves the N-acyl linkage of ceramide into sphingosine and free fatty acid, brain CDase is able to catalyze the reverse reaction, i.e. ceramide synthesis Rattus norvegicus a carboxylate + sphingosine
-
r
3.5.1.23 N-palmitoylsphingosine + H2O
-
Rattus norvegicus palmitic acid + sphingosine
-
r

Synonyms

EC Number Synonyms Comment Organism
3.5.1.23 CDase
-
Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.23 6.5 7 ceramide synthesis activity, narrow optimum in the neutral range Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.5.1.23 5 8 very low ceramide synthesis activity below pH 5 or above pH 8 Rattus norvegicus
3.5.1.23 5.5 10 broad pH-range, ceramidase activity Rattus norvegicus

Cofactor

EC Number Cofactor Comment Organism Structure
3.5.1.23 additional information coenzyme A-independent mechanism Rattus norvegicus