BRENDA - Enzyme Database

Methanopyrus kandleri glutamyl-tRNA reductase

Moser, J.; Lorenz, S.; Hubschwerlen, C.; Rompf, A.; Jahn, D.; J. Biol. Chem. 274, 30679-30685 (1999)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.2.1.70
overexpression in Escherichia coli
Methanopyrus kandleri
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.70
C393S
95% of the GluTR reductase activity compared to wild-type enzyme, 100% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
C42S
no GluTR reductase and GluTR esterase activity
Methanopyrus kandleri
1.2.1.70
C48S
90% of the GluTR reductase activity compared to wild-type enzyme, 95% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
C6S
130% of the GluTR reductase activity compared to wild-type enzyme, 120% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
C90S
85% of the GluTR reductase activity compared to wild-type enzyme, 105% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
H84A
no GluTR reductase activity, 5% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
H84N
30% of the GluTR reductase activity compared to wild-type enzyme, 15% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
5,5'-dithiobis(2-nitrobenzoic acid)
1.0 mM, 90% inhibition
Methanopyrus kandleri
1.2.1.70
glutamate-1-semialdehyde
1.0 mM, 50% inhibition
Methanopyrus kandleri
1.2.1.70
glutamycin
2.5 mM, 75% inhibition
Methanopyrus kandleri
1.2.1.70
heme
0.007 mM, 70% inhibition
Methanopyrus kandleri
1.2.1.70
iodoacetamide
0.01 mM, 30% inhibition, 0.1 mM, complete inhibition
Methanopyrus kandleri
1.2.1.70
N-tosyl-L-phenylalaninechloromethyl ketone
0.1 mM, 90% inhibition, 1.0 mM, complete inhibition
Methanopyrus kandleri
1.2.1.70
PbCl2
0.1 mM, 60% inhibition, 1.0 mM, complete inhibition
Methanopyrus kandleri
1.2.1.70
PtCl4
0.1 mM, 55% inhibition, 1.0 mM, 90% inhibition
Methanopyrus kandleri
1.2.1.70
ZnCl2
0.2 mM, 45% inhibition, 5.0 mM, 90% inhibition
Methanopyrus kandleri
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
45436
-
x * 45436, electrospray ionization mass spectrometry
Methanopyrus kandleri
1.2.1.70
190000
-
gel filtration
Methanopyrus kandleri
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.2.1.70
Methanopyrus kandleri
Q9UXR8
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.2.1.70
-
Methanopyrus kandleri
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
in absence of NADPH, an esterase activity of GluTR hydrolyzes the highly reactive thioester of tRNAGlu to release glutamate
655972
Methanopyrus kandleri
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.70
?
x * 45436, electrospray ionization mass spectrometry
Methanopyrus kandleri
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.70
90
-
-
Methanopyrus kandleri
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.70
8.1
-
-
Methanopyrus kandleri
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
additional information
the enzyme does not possess a chromophoric prosthetic group
Methanopyrus kandleri
1.2.1.70
NADPH
-
Methanopyrus kandleri
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.2.1.70
Methanopyrus kandleri
isoelectric focusing
-
6
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
overexpression in Escherichia coli
Methanopyrus kandleri
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.70
additional information
the enzyme does not possess a chromophoric prosthetic group
Methanopyrus kandleri
1.2.1.70
NADPH
-
Methanopyrus kandleri
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.2.1.70
C393S
95% of the GluTR reductase activity compared to wild-type enzyme, 100% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
C42S
no GluTR reductase and GluTR esterase activity
Methanopyrus kandleri
1.2.1.70
C48S
90% of the GluTR reductase activity compared to wild-type enzyme, 95% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
C6S
130% of the GluTR reductase activity compared to wild-type enzyme, 120% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
C90S
85% of the GluTR reductase activity compared to wild-type enzyme, 105% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
H84A
no GluTR reductase activity, 5% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
1.2.1.70
H84N
30% of the GluTR reductase activity compared to wild-type enzyme, 15% of the GluTR esterase activity compared to wild-type enzyme
Methanopyrus kandleri
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.2.1.70
5,5'-dithiobis(2-nitrobenzoic acid)
1.0 mM, 90% inhibition
Methanopyrus kandleri
1.2.1.70
glutamate-1-semialdehyde
1.0 mM, 50% inhibition
Methanopyrus kandleri
1.2.1.70
glutamycin
2.5 mM, 75% inhibition
Methanopyrus kandleri
1.2.1.70
heme
0.007 mM, 70% inhibition
Methanopyrus kandleri
1.2.1.70
iodoacetamide
0.01 mM, 30% inhibition, 0.1 mM, complete inhibition
Methanopyrus kandleri
1.2.1.70
N-tosyl-L-phenylalaninechloromethyl ketone
0.1 mM, 90% inhibition, 1.0 mM, complete inhibition
Methanopyrus kandleri
1.2.1.70
PbCl2
0.1 mM, 60% inhibition, 1.0 mM, complete inhibition
Methanopyrus kandleri
1.2.1.70
PtCl4
0.1 mM, 55% inhibition, 1.0 mM, 90% inhibition
Methanopyrus kandleri
1.2.1.70
ZnCl2
0.2 mM, 45% inhibition, 5.0 mM, 90% inhibition
Methanopyrus kandleri
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.2.1.70
45436
-
x * 45436, electrospray ionization mass spectrometry
Methanopyrus kandleri
1.2.1.70
190000
-
gel filtration
Methanopyrus kandleri
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.70
-
Methanopyrus kandleri
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.2.1.70
L-glutamyl-tRNAGlu + NADPH + H+
in absence of NADPH, an esterase activity of GluTR hydrolyzes the highly reactive thioester of tRNAGlu to release glutamate
655972
Methanopyrus kandleri
L-glutamate 1-semialdehyde + NADP+ + tRNAGlu
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.70
?
x * 45436, electrospray ionization mass spectrometry
Methanopyrus kandleri
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.70
90
-
-
Methanopyrus kandleri
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.70
8.1
-
-
Methanopyrus kandleri
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
1.2.1.70
Methanopyrus kandleri
isoelectric focusing
-
6