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Literature summary extracted from

  • Nakamura, T.; Takagaki, K.; Majima, M.; Kimura, S.; Kubo, K.; Endo, M.
    A new type of exo-beta-glucuronidase acting only on non-sulfated glycosaminoglycans (1990), J. Biol. Chem., 265, 5390-5397.
    View publication on PubMed

General Stability

EC Number General Stability Organism
3.2.1.31 the enzyme acting on chondroitin is less stable than enzyme acting on p-nitrophenylphosphate Oryctolagus cuniculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.31 NaCl between 0.075 and 0.25 M, the activity is decreased with increasing NaCl concentration. Above 0.25 M, the enzyme shows no activity. The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition Oryctolagus cuniculus
3.2.1.31 saccharo-1,4-lactone
-
 Oryctolagus cuniculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.31 6.3
-
 chondroitin pH 5.0, 37°C Oryctolagus cuniculus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.31 NaCl below 0.075 M the activity of beta-glucuronidase acting on chondroitin is increased with increasing NaCl concentrations.The activity of beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide is decreased with increasing NaCl concentrations, and 0.4 M NaCl produces 50% inhibition Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.31 75000
-
 x * 75000, SDS-PAGE, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus
3.2.1.31 130000
-
 gel filtration, beta-glucuronidase acting on chondroitin Oryctolagus cuniculus
3.2.1.31 280000 300000 gel filtration, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.31 Oryctolagus cuniculus
-
 2 different exo-beta-glucuronidases: a beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide and a beta-glucuronidase acting on chondroitin
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.31 2 different exo-beta-glucuronidases: a beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide and a beta-glucuronidase acting on chondroitin Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.31 liver
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.31 0.127
-
 beta-glucuronidase acting on chondroitin Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.2.1.31 -20°C, enzyme acting on chondroitin retains high level of activity for at least 1 month Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.31 (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2 + H2O no activity with enzyme acting on chondroitin, 142% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3 + H2O no activity with enzyme acting on chondroitin, 63% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2 + H2O no activity with enzyme acting on chondroitin, 50% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3 + H2O no activity with enzyme acting on chondroitin, 33% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 (4GlcAbeta1-3GalNAcbeta1-)2 + H2O 66% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 90% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 (4GlcAbeta1-3GalNAcbeta1-)3 + H2O 56% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 71% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 (4GlcAbeta1-3GlcNAcbeta1-)3 + H2O 81% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 73% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 chondroitin + H2O MW 3500 Da: 23% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 41% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 8000 Da: 11% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 28% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide. MW 15000 Da: 6% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 9% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?
3.2.1.31 GlcAbeta1-3GalNAc(4-OSO3-) + H2O no activity with enzyme acting on chondroitin, 4% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + N-acetylamino-beta-D-galactopyranose 4-sulfate
-
 ?
3.2.1.31 additional information no activity with enzyme acting on chondroitin: N-acetylhyalobiuronic acid, N-acetylchondrosine, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(4-OSO3-)beta1-)2, GlcAbeta1-3galNAc(4-OSO3-), (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)3, (4GlcAbeta1-3GalNAc(6-OSO3-)beta1-)2. No activity with enzyme acting on p-nitrophenyl-beta-D-glucuronide: N-acetylhyalobiuronic acid, N-acetylchondrosine Oryctolagus cuniculus ?
-
 ?
3.2.1.31 p-nitrophenyl-beta-D-glucuronide + H2O 1% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on chondroitin, 192% of the activity with (4GlcAbeta1-3GlcNAc1-)2 with the enzyme acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus D-glucuronate + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.31 ? x * 75000, SDS-PAGE, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus
3.2.1.31 More beta-glucuronidase acting on chondroitin yields several protein bands on SDS-PAGE, no protein band with a MW of 75000 Da Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.31 exo-beta-glucuronidase
-
 Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.31 4.5
-
 beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus
3.2.1.31 5
-
 beta-glucuronidase acting on chondroitin Oryctolagus cuniculus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.31 3 5 pH 3.0: about 50% of maximal activity, pH 5.0: about 70% of maximal activity, beta-glucuronidase acting on p-nitrophenyl-beta-D-glucuronide Oryctolagus cuniculus
3.2.1.31 4 5.5 pH 4.0: about 45% of maximal activity, pH 5.5: about 90% of maximal activity, pH 6.0: no activity, beta-glucuronidase acting on chondroitin Oryctolagus cuniculus