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Literature summary extracted from

  • Pilly, D.; Niemeyer, A.; Schmidt, M.; Bargetzi, J.P.
    Enzymes for RNA sequence analysis. Preparation and specificity of exoplasmodial ribonucleases I and II from Physarum polycephalum (1978), J. Biol. Chem., 253, 437-445.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.1.26.1 extracellular secretion, both enzyme forms Physarum polycephalum
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.26.1 24500
-
 RNase Phy II, gel filtration Physarum polycephalum
3.1.26.1 25000
-
 RNase Phy I, gel filtration Physarum polycephalum

Organism

EC Number Organism UniProt Comment Textmining
3.1.26.1 Physarum polycephalum
-
 2 enzyme forms: Rnase Phy I and RNase Phy II
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.26.1 RNase Phy I and II Physarum polycephalum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.26.1 additional information submersed culture Physarum polycephalum
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.26.1 ApA + H2O
-
 Physarum polycephalum 5'-AMP + adenosine
-
 ?
3.1.26.1 ApC + H2O
-
 Physarum polycephalum 5'-CMP + adenosine
-
 ?
3.1.26.1 ApG + H2O
-
 Physarum polycephalum 5'-GMP + adenosine
-
 ?
3.1.26.1 ApU + H2O
-
 Physarum polycephalum 5'-UMP + adenosine
-
 ?
3.1.26.1 CpA + H2O
-
 Physarum polycephalum 5'-AMP + cytidine
-
 ?
3.1.26.1 CpC + H2O
-
 Physarum polycephalum 5'-CMP + cytidine
-
 ?
3.1.26.1 CpG + H2O
-
 Physarum polycephalum 5'-GMP + cytidine
-
 ?
3.1.26.1 CpU + H2O
-
 Physarum polycephalum 5'-UMP + cytidine
-
 ?
3.1.26.1 GpA + H2O
-
 Physarum polycephalum 5'-AMP + guanosine
-
 ?
3.1.26.1 GpC + H2O
-
 Physarum polycephalum 5'-CMP + guanosine
-
 ?
3.1.26.1 GpG + H2O
-
 Physarum polycephalum 5'-GMP + guanosine
-
 ?
3.1.26.1 GpU + H2O
-
 Physarum polycephalum 5'-UMP + guanosine
-
 ?
3.1.26.1 additional information substrate specificity of RNase Phy I and II, the latter shows a preference for GpN substrates, while rejecting ApN dinucleotide substrates, RNase I shows a preference for ApN before GpN substrate, while CpN dinucleotides are rather resistant against hydrolysis, overview Physarum polycephalum ?
-
 ?
3.1.26.1 RNA + H2O low molecular weigt yeast RNA Physarum polycephalum 5'-phosphonucleotide product identification ?
3.1.26.1 UpA + H2O
-
 Physarum polycephalum 5'-AMP + uridine
-
 ?
3.1.26.1 UpC + H2O
-
 Physarum polycephalum 5'-CMP + uridine
-
 ?
3.1.26.1 UpG + H2O
-
 Physarum polycephalum 5'-GMP + uridine
-
 ?
3.1.26.1 UpU + H2O
-
 Physarum polycephalum 5'-UMP + uridine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.26.1 40
-
 RNase Phy I and II, assay at Physarum polycephalum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.26.1 3.1
-
 RNase Phy II, assay at Physarum polycephalum
3.1.26.1 4.5
-
 RNase Phy II, assay at Physarum polycephalum

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.1.26.1 8
-
 nactivation, RNase Phy I and II Physarum polycephalum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.26.1 Physarum polycephalum RNase Phy I, isoelectric focusing
-
 4.2
3.1.26.1 Physarum polycephalum RNase Phy II, isoelectric focusing
-
 4.2