EC Number | Cloned (Comment) | Organism |
---|---|---|
3.2.1.20 | DNA and amino acid sequence determination and analysis, expression in Escherichia coli | Thermus caldophilus |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 60000 | - |
x * 60000, SDS-PAGE | Thermus caldophilus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.2.1.20 | Thermus caldophilus | - |
enzyme TcaAG | - |
3.2.1.20 | Thermus caldophilus GK24 | - |
enzyme TcaAG | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.2.1.20 | 78fold to homogeneity | Thermus caldophilus |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.2.1.20 | maltotetraose + 3 H2O = 4 alpha-D-glucose | residues Asp198, Glu265, and Asp327 are involved in catalysis, His100 and His326 are involved in substrate binding | Thermus caldophilus |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 999.5 | - |
purified enzyme, substrate 4-nitrophenyl alpha-D-glucopyranoside | Thermus caldophilus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.2.1.20 | 4-methylumbelliferyl alpha-D-glucopyranoside + H2O | - |
Thermus caldophilus | 4-methylumbelliferol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | 4-methylumbelliferyl alpha-D-glucopyranoside + H2O | - |
Thermus caldophilus GK24 | 4-methylumbelliferol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Thermus caldophilus | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | - |
Thermus caldophilus GK24 | 4-nitrophenol + alpha-D-glucopyranose | - |
? | |
3.2.1.20 | isomaltosaccharides + H2O | hydrolysis of alpha-1,6-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus | alpha-D-glucose | - |
? | |
3.2.1.20 | additional information | no activity with cellobiose, laminaribiose, trehalose, strach, pullulan, lactose, maltose, leucrose, and gentibiose | Thermus caldophilus | ? | - |
? | |
3.2.1.20 | additional information | no activity with cellobiose, laminaribiose, trehalose, strach, pullulan, lactose, maltose, leucrose, and gentibiose | Thermus caldophilus GK24 | ? | - |
? | |
3.2.1.20 | nigerose + H2O | hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus | 2 alpha-D-glucose | - |
? | |
3.2.1.20 | nigerose + H2O | hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus GK24 | 2 alpha-D-glucose | - |
? | |
3.2.1.20 | panose + H2O | hydrolysis of alpha-1,6-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus | alpha-D-glucopyranose + alpha-D-glucopyranosyl-1,4-alpha-D-glucopyranosyl-1,6-alpha-D-glucopyranosyl-1,4-D-glucopyranose | - |
? | |
3.2.1.20 | sucrose + H2O | hydrolysis of alpha-1,2-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus | alpha-D-glucose + D-fructose | - |
? | |
3.2.1.20 | turanose + H2O | hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus | alpha-D-glucose + D-fructose | - |
? | |
3.2.1.20 | turanose + H2O | hydrolysis of alpha-1,3-glycosidic bond of the nonreducing terminal unit | Thermus caldophilus GK24 | alpha-D-glucose + D-fructose | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.2.1.20 | ? | x * 60000, SDS-PAGE | Thermus caldophilus |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.2.1.20 | TcaAG | - |
Thermus caldophilus |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 90 | - |
- |
Thermus caldophilus |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 90 | - |
stable up to, half-life 85 min | Thermus caldophilus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 6.5 | - |
- |
Thermus caldophilus |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
3.2.1.20 | 6 | 8.5 | stable | Thermus caldophilus |