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Literature summary extracted from

  • Igarashi, S.; Sode, K.
    Construction and characterization of heterodimeric soluble quinoprotein glucose dehydrogenase (2004), J. Biochem. Biophys. Methods, 61, 331-338.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.5.2 expression of isozyme PQQGDH-B with and without an polyarginine tail in Escherichia coli strain PP2418, and of mutant H168Q Acinetobacter calcoaceticus
1.1.99.35
-
 Acinetobacter calcoaceticus

Protein Variants

EC Number Protein Variants Comment Organism
1.1.5.2 H168Q site-directed mutagenesis, inactive mutant, a heterodimeric chimeric enzyme consisiting of 1 wild-type subunit and 1 mutant subunit shows decreased activity and a substrate specificity similar to the wild-type enzyme Acinetobacter calcoaceticus
1.1.99.35 additional information constructed of heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits. The heterodimeric wild-type/H168Q shows slightly decreased GDH activity and almost identical substrate specificity profile to the wild-type enzyme. The Hill coefficient of the heterodimer is calculated as 1.13, indicating positive cooperativity Acinetobacter calcoaceticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.5.2 additional information
-
 additional information kinetics, cooperativity in the recombinant chimeric mutant enzyme which possesses only 1 active subunit derived from the wild-type enzyme Acinetobacter calcoaceticus
1.1.5.2 2 3 D-glucose recombinant Arg-tagged enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 2 3 3-O-methyl-D-glucose recombinant Arg-tagged enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 8
-
 D-galactose recombinant Arg-tagged enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 9
-
 D-galactose recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 13
-
 maltose recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 14
-
 maltose recombinant Arg-tagged enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 20
-
 lactose recombinant Arg-tagged enzyme, and recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 22
-
 D-glucose recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 22
-
 3-O-methyl-D-glucose recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 34
-
 allose recombinant Arg-tagged enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 36
-
 allose recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 2 3 D-glucose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 2 3 3-O-methyl-D-glucose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 8
-
 D-galactose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 9
-
 D-galactose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 13
-
 maltose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 14
-
 maltose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 20
-
 lactose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 20
-
 lactose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 22
-
 D-glucose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 22
-
 3-O-methyl-D-glucose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 34
-
 D-allose recombinant wild-type with R4-tail, pH 7.0 Acinetobacter calcoaceticus
1.1.99.35 36
-
 D-allose heterodimer wild-type/mutant H168Q, pH 7.0 Acinetobacter calcoaceticus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.1.5.2 soluble isozyme PQQGDH-B Acinetobacter calcoaceticus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.5.2 Ca2+
-
 Acinetobacter calcoaceticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.5.2 50000
-
 2 * 50000, SDS-PAGE Acinetobacter calcoaceticus

Organism

EC Number Organism UniProt Comment Textmining
1.1.5.2 Acinetobacter calcoaceticus
-
 isozyme PQQGDH-B
-
1.1.99.35 Acinetobacter calcoaceticus
-
-
-

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.1.5.2 protein dissociation and redimerization of recombinant wild-type and Arg-tagged enzyme, and the mutant H168Q, overview Acinetobacter calcoaceticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.5.2 2642
-
 purified recombinant mutant chimeric isozyme PQQGDH-B Acinetobacter calcoaceticus
1.1.5.2 4512
-
 purified recombinant Arg-tagged isozyme PQQGDH-B Acinetobacter calcoaceticus
1.1.5.2 4610
-
 purified recombinant wild-type isozyme PQQGDH-B Acinetobacter calcoaceticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.5.2 3-O-methyl-D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 allose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 D-galactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ?
-
 ?
1.1.5.2 D-glucose + ubiquinone
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + ubiquinol
-
 ?
1.1.5.2 lactose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.5.2 maltose + 2,6-dichlorophenolindolphenol
-
 Acinetobacter calcoaceticus ?
-
 ?
1.1.99.35 3-O-methyl-D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus 3-O-methyl-D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-allose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-allono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-galactose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-galactono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 D-glucose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus D-glucono-1,5-lactone + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 lactose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
 ?
1.1.99.35 maltose + oxidized 2,6-dichlorophenolindophenol
-
 Acinetobacter calcoaceticus ? + reduced 2,6-dichlorophenolindophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.5.2 dimer 2 * 50000, SDS-PAGE Acinetobacter calcoaceticus
1.1.99.35 dimer study on heterodimeric PQQGDH-B composed of native wild-type and inactive mutant H168Q subunits Acinetobacter calcoaceticus

Synonyms

EC Number Synonyms Comment Organism
1.1.5.2 glucose dehydrogenase
-
 Acinetobacter calcoaceticus
1.1.5.2 PQQGDH-B
-
 Acinetobacter calcoaceticus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.5.2 3778
-
 D-glucose recombinant Arg-tagged enzyme, pH 7.0 Acinetobacter calcoaceticus
1.1.5.2 4424
-
 D-glucose recombinant chimeric mutant enzyme, pH 7.0 Acinetobacter calcoaceticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.5.2 7
-
 assay at Acinetobacter calcoaceticus

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.5.2 pyrroloquinoline quinone i.e. PQQ, dependent on Acinetobacter calcoaceticus