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Literature summary extracted from

  • Narahara, E.; Makino, Y.; Omichi, K.
    Glycogen debranching enzyme in bovine brain (2001), J. Biochem., 130, 465-470.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.33 AMP 10 mM, 86% of initial activity Bos taurus
3.2.1.33 ATP 10 mM, no activity Bos taurus
3.2.1.33 Cd2+ 1 mM, no activity Bos taurus
3.2.1.33 Co2+ 1 mM, 15% of initial activity Bos taurus
3.2.1.33 Cu2+ 1 mM, 6% of initial activity Bos taurus
3.2.1.33 D-glucose 10 mM, 89% of initial activity Bos taurus
3.2.1.33 D-glucose-1-phosphate 10 mM, 82% of initial activity Bos taurus
3.2.1.33 D-Glucose-6-phosphate 10 mM, 89% of initial activity Bos taurus
3.2.1.33 Fe2+ 1 mM, 7% of initial activity Bos taurus
3.2.1.33 Hg2+ 1 mM, 3% of initial activity Bos taurus
3.2.1.33 Ni2+ 1 mM, 25% of initial activity Bos taurus
3.2.1.33 Zn2+ 1 mM, 4% of initial activity Bos taurus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.33 cytosol
-
 Bos taurus 5829
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.33 Ba2+ 1 mM, 154% of initial activity Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.33 190000
-
 gel filtration Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.33 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.33 partial Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.33 brain cerebrum Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.33 3100
-
 37°C, pH 6.5 Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.33 Glc1-4Glc-alpha1-4(Glc-alpha1-6)Glc-alpha1-4 Glc-alpha1-4 Glc-alpha1-4Glc-2-aminopyridine + H2O
-
 Bos taurus ?
-
 ?
3.2.1.33 additional information no alpha-glucosidase or alpha-amylase activity Bos taurus ?
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.33 6
-
-
 Bos taurus