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Literature summary extracted from
- Crystal structure of Bacillus stearothermophilus alpha-amylase: possible factors determining the thermostability (2001), J. Biochem., 129, 461-468.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | expression in Bacillus subtilis | Geobacillus stearothermophilus |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | tiny needle crystals are formed in 35 mM Na-acetate, pH 4.6, 35 mM CaCl2, 6.25% 2-propanol in the presence of 1.23% of the pseudo-oligosaccharide inhibitor acarbose at a protein concentration of 10 mg/ml, crystals diffract to 2.0 A resolution | Geobacillus stearothermophilus |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | alpha-1,4-glucan + H2O | Geobacillus stearothermophilus | - |
fragments of alpha-1,4-glucan | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.1 | Geobacillus stearothermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.1 | recombinant alpha-amylase | Geobacillus stearothermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.1 | alpha-1,4-glucan + H2O | - |
Geobacillus stearothermophilus | fragments of alpha-1,4-glucan | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.1 | BSTA | - |
Geobacillus stearothermophilus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 75 | - |
- |
Geobacillus stearothermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 90 | - |
50% loss of activity after 50 min at pH 6.5 | Geobacillus stearothermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.1 | 6.5 | - |
- |
Geobacillus stearothermophilus |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.1 | Geobacillus stearothermophilus | - |
- |
6.9 |
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Release 2023.1 (January 2023)
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