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Literature summary extracted from
- Purification and properties of recombinant Plasmodium falciparum S-adenosyl-L-homocysteine hydrolase (2001), J. Biochem., 129, 101-105.
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | medicine | development of a specific inhibitor for Plasmodium falciparum SAH hydrolase as a chemotherapeutic agent against malaria | Plasmodium falciparum |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | expression in Escherichia coli | Homo sapiens |
| 3.13.2.1 | expression in Escherichia coli | Plasmodium falciparum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | neplanocin A | IC50: 47 nM | Homo sapiens |  |
| 3.13.2.1 | neplanocin A | IC50: 101 nM | Plasmodium falciparum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.0006 | - |
DL-homocysteine | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.13.2.1 | 0.0012 | - |
S-adenosyl-DL-homocysteine | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.13.2.1 | 0.0014 | - |
DL-homocysteine | pH 7.2, 30°C | Homo sapiens | |
| 3.13.2.1 | 0.0078 | - |
S-adenosyl-DL-homocysteine | pH 7.2, 30°C | Homo sapiens | |
| 3.13.2.1 | 0.0643 | - |
adenosine | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.13.2.1 | 0.111 | - |
adenosine | pH 7.2, 30°C | Homo sapiens | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 43000 | - |
4 * 43000, SDS-PAGE | Homo sapiens |
| 3.13.2.1 | 55000 | - |
4 * 55000, SDS-PAGE | Plasmodium falciparum |
| 3.13.2.1 | 150000 | - |
gel filtration | Homo sapiens |
| 3.13.2.1 | 200000 | - |
gel filtration | Plasmodium falciparum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.13.2.1 | Homo sapiens | - |
- |
- |
| 3.13.2.1 | Plasmodium falciparum | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.13.2.1 | - |
Homo sapiens |
| 3.13.2.1 | - |
Plasmodium falciparum |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 0.11 | - |
- |
Plasmodium falciparum |
| 3.13.2.1 | 2.4 | - |
- |
Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.13.2.1 | S-adenosyl-DL-homocysteine + H2O | - |
Homo sapiens | DL-homocysteine + adenosine | - |
r | |
| 3.13.2.1 | S-adenosyl-DL-homocysteine + H2O | - |
Plasmodium falciparum | DL-homocysteine + adenosine | - |
r | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | tetramer | 4 * 43000, SDS-PAGE | Homo sapiens |
| 3.13.2.1 | tetramer | 4 * 55000, SDS-PAGE | Plasmodium falciparum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.13.2.1 | SAH hydrolase | - |
Homo sapiens |
| 3.13.2.1 | SAH hydrolase | - |
Plasmodium falciparum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 50 | - |
hydrolysis of S-adenosyl-DL-homocysteine | Homo sapiens |
| 3.13.2.1 | 55 | - |
hydrolysis of S-adenosyl-DL-homocysteine | Plasmodium falciparum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 35 | 55 | 35°C: about 60% of maximal activity, 55°C: about 75% of maximal activity, hydrolysis of S-adenosyl-DL-homocysteine | Homo sapiens |
| 3.13.2.1 | 40 | 60 | 40°C: about 50% of maximal activity, 60°C: about 85% of maximal activity, hydrolysis of S-adenosyl-DL-homocysteine | Plasmodium falciparum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 84 | - |
S-adenosyl-DL-homocysteine | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.13.2.1 | 312 | - |
adenosine | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.13.2.1 | 312 | - |
DL-homocysteine | pH 7.2, 30°C | Plasmodium falciparum | |
| 3.13.2.1 | 1908 | - |
S-adenosyl-DL-homocysteine | pH 7.2, 30°C | Homo sapiens | |
| 3.13.2.1 | 6666 | - |
adenosine | pH 7.2, 30°C | Homo sapiens | |
| 3.13.2.1 | 6666 | - |
DL-homocysteine | pH 7.2, 30°C | Homo sapiens | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 7.4 | - |
hydrolysis of S-adenosyl-DL-homocysteine | Homo sapiens |
| 3.13.2.1 | 7.6 | - |
hydrolysis of S-adenosyl-DL-homocysteine | Plasmodium falciparum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.13.2.1 | 6 | 8 | pH 6.0: about 45% of maximal activity, pH 8.0: about 90% of maximal activity, hydrolysis of S-adenosyl-DL-homocysteine | Plasmodium falciparum |
| 3.13.2.1 | 6 | 8 | pH 6.0: about 60% of maximal activity, pH 8.0: about 60% of maximal activity, hydrolysis of S-adenosyl-DL-homocysteine | Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.13.2.1 | NAD+ | contains 0.27 mol of NAD+ per mol of subunit | Plasmodium falciparum | |
| 3.13.2.1 | NAD+ | contains 0.65 mol of NAD+ per mol of subunit | Homo sapiens | |
| 3.13.2.1 | NADH | contains 0.19 mol of NAD+ per mol of subunit | Homo sapiens | |
| 3.13.2.1 | NADH | contains 0.47 mol of NAD+ per mol of subunit | Plasmodium falciparum | |
IC50 Value
| EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
|---|---|---|---|---|---|---|
| 3.13.2.1 | 0.000047 | - |
IC50: 47 nM | Homo sapiens | neplanocin A | |
| 3.13.2.1 | 0.000101 | - |
IC50: 101 nM | Plasmodium falciparum | neplanocin A | |
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