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Literature summary extracted from

  • Ho, K.K.; Weiner, H.
    Isolation and characterization of an aldehyde dehydrogenase encoded by the aldB gene of Escherichia coli (2005), J. Bacteriol., 187, 1067-1073.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.4 ethanol 0.5-2% increases activity in crude extracts Escherichia coli

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.4 native and mutant protein expressed in Escherichia coli DH5alpha Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.4 R197E very low activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.4 0.0025
-
acetaldehyde pH 7.5, 25°C Escherichia coli
1.2.1.4 0.0036
-
Chloroacetaldehyde pH 7.5, 25°C Escherichia coli
1.2.1.4 0.0058
-
propanal pH 7.5, 25°C Escherichia coli
1.2.1.4 0.056
-
benzaldehyde pH 7.5, 25°C Escherichia coli
1.2.1.4 0.1
-
NADP+ pH 7.5, 25°C Escherichia coli
1.2.1.4 0.9
-
4-hydroperoxycyclophosphamide pH 7.5, 25°C Escherichia coli

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.2.1.4 Mg2+ stimulates activity by about 100% Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.4 56352
-
4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry Escherichia coli
1.2.1.4 58000
-
4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry Escherichia coli
1.2.1.4 220000
-
gel filtration Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.4 acetaldehyde + NADP+ + H2O Escherichia coli
-
acetate + NADPH + H+
-
?
1.2.1.4 an aldehyde + NADP+ + H2O Escherichia coli removal of aldehydes and alcohols in cells under stress an acid + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.4 Escherichia coli
-
strain ATCC 700926D, highest activity in cells grown with ethanol
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.4
-
Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.2.1.4 2
-
purified enzyme, pH 7.5, 25°C Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.4 4-hydroperoxycyclophosphamide + NADP+ low activity Escherichia coli ?
-
?
1.2.1.4 acetaldehyde + NADP+ + H2O
-
Escherichia coli acetate + NADPH + H+
-
?
1.2.1.4 an aldehyde + NADP+ + H2O removal of aldehydes and alcohols in cells under stress Escherichia coli an acid + NADPH + H+
-
?
1.2.1.4 benzaldehyde + NADP+ l40% of the activity with acetaldehyde Escherichia coli benzoate + NADPH
-
?
1.2.1.4 chloroacetaldehyde + NADP+ + H2O most effective substrate in the absence of Mg2+ Escherichia coli chloroacetate + NADPH + H+
-
?
1.2.1.4 mafosfamide + NADP+ low activity Escherichia coli ?
-
?
1.2.1.4 additional information no activity with glycolaldehyde and glyceraldehyde Escherichia coli ?
-
?
1.2.1.4 propanal + NADP+ + H2O much lower activity than with acetaldehyde Escherichia coli propionate + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.2.1.4 tetramer 4 * 58000, SDS-PAGE, 4 * 56352, mass spectrometry Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.4 7
-
-
Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.4 additional information no activity with NAD+ Escherichia coli
1.2.1.4 NADP+
-
Escherichia coli