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Literature summary extracted from

  • Johnsen, U.; Schonheit, P.
    Novel xylose dehydrogenase in the halophilic archaeon Haloarcula marismortui (2004), J. Bacteriol., 186, 6198-6207.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.179 overexpression in Escherichia coli Haloarcula marismortui

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.179 1.2
-
D-xylose pH 8.3, 37°C Haloarcula marismortui
1.1.1.179 2.3
-
D-ribose pH 8.3, 37°C Haloarcula marismortui
1.1.1.179 64
-
D-glucose pH 8.3, 37°C Haloarcula marismortui

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.179 KCl maximal activity at 1.5 M Haloarcula marismortui
1.1.1.179 MgCl2 maximal activity at 100 M Haloarcula marismortui
1.1.1.179 NaCl maximal activity at 1.5 M Haloarcula marismortui

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.179 39900
-
4 * 39900, SDS-PAGE Haloarcula marismortui
1.1.1.179 175000
-
gel filtration Haloarcula marismortui

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.179 D-xylose + NADP+ Haloarcula marismortui initial step in xylose degradation D-xylonolactone + NADPH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.179 Haloarcula marismortui Q5UY95
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.179
-
Haloarcula marismortui

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.179 culture condition:xylose-grown cell
-
Haloarcula marismortui
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.179 100
-
pH 8.8, 37°C Haloarcula marismortui

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.179 D-glucose + NADP+ reaction is catalyzed with 70fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP* Haloarcula marismortui D-gluconolactone + NADPH + H+
-
?
1.1.1.179 D-ribose + NADP+ reaction is catalyzed with 1.8fold lower catalytic efficiency (kcat/KM) compared to activity with D-xylose + NADP* Haloarcula marismortui D-ribonolactone + NADPH + H+
-
?
1.1.1.179 D-xylose + NAD+ Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui D-xylonolactone + NADH + H+
-
?
1.1.1.179 D-xylose + NADP+ initial step in xylose degradation Haloarcula marismortui D-xylonolactone + NADPH + H+
-
?
1.1.1.179 D-xylose + NADP+ Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui D-xylonolactone + NADPH + H+
-
?

Subunits

EC Number Subunits Comment Organism
1.1.1.179 homotetramer 4 * 39900, SDS-PAGE Haloarcula marismortui

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.179 37
-
assay at Haloarcula marismortui
1.1.1.179 50
-
-
Haloarcula marismortui

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.179 25 50 activity increases exponentially with temperature between 25°C and 45°C. 50°C: optimum Haloarcula marismortui

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.179 273
-
D-glucose pH 8.3, 37°C Haloarcula marismortui
1.1.1.179 356
-
D-xylose pH 8.3, 37°C Haloarcula marismortui
1.1.1.179 360
-
D-ribose pH 8.3, 37°C Haloarcula marismortui

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.179 8.3
-
-
Haloarcula marismortui

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.179 7 9 more than 50% of maximal activity Haloarcula marismortui

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.179 NAD+ Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui
1.1.1.179 NADP+ preferred electron acceptor. Vmax/Km for NADP+ is 7fold higher than Vmax/KM for NAD+ Haloarcula marismortui

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.1.1.179 0.043
-
D-glucose pH 8.3, 37°C Haloarcula marismortui
1.1.1.179 1.57
-
D-ribose pH 8.3, 37°C Haloarcula marismortui
1.1.1.179 2.97
-
D-xylose pH 8.3, 37°C Haloarcula marismortui