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Literature summary extracted from

  • Hildmann, C.; Ninkovic, M.; Dietrich, R.; Wegener, D.; Riester, D.; Zimmermann, T.; Birch, O.M.; Bernegger, C.; Loidl, P.; Schwienhorst, A.
    A new amidohydrolase from Bordetella or Alcaligenes strain FB188 with similarities to histone deacetylases (2004), J. Bacteriol., 186, 2328-2339.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.5.1.4 expression in Escherichia coli Alcaligenes sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.5.1.4 suberoylanilide hydroxamic acid strong inhibition Alcaligenes sp.
3.5.1.4 tricostatin A strong inhibition Alcaligenes sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.5.1.4 0.04
-
 acetate-prelabeled chicken histones
-
 Alcaligenes sp.
3.5.1.4 0.127
-
 Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) pH 7.5, 37°C Alcaligenes sp.
3.5.1.4 4
-
 cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide pH 7.5, 37°C Alcaligenes sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.5.1.4 Ca2+ activation above 100 mM Alcaligenes sp.
3.5.1.4 Co2+ increases 1.3fold the activity at 1 mM, inhibition at higher concentration Alcaligenes sp.
3.5.1.4 Mg2+ activation above 100 mM Alcaligenes sp.
3.5.1.4 Mn2+ activation above 100 mM Alcaligenes sp.
3.5.1.4 Ni2+ increases 1.1fold the activity at 1 mM, inhibition at higher concentration Alcaligenes sp.
3.5.1.4 Zn2+ required for activity Alcaligenes sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.5.1.4 39420
-
 from amino acid sequence Alcaligenes sp.

Organism

EC Number Organism UniProt Comment Textmining
3.5.1.4 Alcaligenes sp. Q70I53 strain FB188, or Bordetella sp.
-
3.5.1.4 Alcaligenes sp. DSM 11172 Q70I53 strain FB188, or Bordetella sp.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.5.1.4 several chromatographic steps, including Q-Sepharose and Sephacryl S-100 Alcaligenes sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.5.1.4 0.004
-
 acetylspermidine, pH 7.5 Alcaligenes sp.
3.5.1.4 0.008
-
 acetylcadaverine, pH 7.5 Alcaligenes sp.
3.5.1.4 0.011
-
 acetylputrescine, pH 7.5 Alcaligenes sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.5.1.4 acetate-prelabeled chicken histones + H2O
-
 Alcaligenes sp. deacetylated chicken histones + acetate
-
 ?
3.5.1.4 acetate-prelabeled chicken histones + H2O
-
 Alcaligenes sp. DSM 11172 deacetylated chicken histones + acetate
-
 ?
3.5.1.4 acetylcadaverine + H2O
-
 Alcaligenes sp. acetate + cadaverine
-
 ?
3.5.1.4 acetylcadaverine + H2O
-
 Alcaligenes sp. DSM 11172 acetate + cadaverine
-
 ?
3.5.1.4 acetylputrescine + H2O
-
 Alcaligenes sp. acetate + putrescine
-
 ?
3.5.1.4 acetylputrescine + H2O
-
 Alcaligenes sp. DSM 11172 acetate + putrescine
-
 ?
3.5.1.4 acetylspermidine + H2O
-
 Alcaligenes sp. acetate + spermidine
-
 ?
3.5.1.4 acetylspermidine + H2O
-
 Alcaligenes sp. DSM 11172 acetate + spermidine
-
 ?
3.5.1.4 Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
-
 Alcaligenes sp. Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
-
 ?
3.5.1.4 Boc-Lys(epsilon-acetyl)-(4-methyl-coumaryl-7-amide) + H2O
-
 Alcaligenes sp. DSM 11172 Boc-Lys-(4-methyl-coumaryl-7-amide) + acetate
-
 ?
3.5.1.4 cis-(1S,4R)-N-(4-(hydroxymethyl)cyclopent-2-enyl)acetamide + H2O used as enzymatic assay Alcaligenes sp. cis-(1S,4R)-(4-(hydroxymethyl)cyclopent-2-enyl)amine + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.5.1.4 monomer 1* 35000, SDS-PAGE Alcaligenes sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.5.1.4 8
-
-
 Alcaligenes sp.