Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Giladi, M.; Altman-Price, N.; Levin, I.; Levy, L.; Mevarech, M.
    FolM, a new chromosomally encoded dihydrofolate reductase in Escherichia coli (2003), J. Bacteriol., 185, 7015-7018.
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.5.1.3 methotrexate
-
 Escherichia coli
1.5.1.3 additional information no inhibition with trimethoprim up to 1.38 mM Escherichia coli
1.5.1.50 additional information not inhibitory: trimethoprim Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.3 0.0019
-
 NADPH pH 6.0 Escherichia coli
1.5.1.3 0.0095
-
 7,8-dihydrofolate pH 6.0 Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.3 Escherichia coli
-
-
-
1.5.1.50 Escherichia coli P0AFS3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.3
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.3 7,8-dihydrofolate + NADPH cannot use NADH as reducing agent Escherichia coli 5,6,7,8-tetrahydrofolate + NADP+
-
 ?
1.5.1.3 dihydrobiopterin + NADPH 10% of the activity with 7,8-dihydrofolate Escherichia coli ? + NADP+
-
 ?
1.5.1.50 additional information no substrate: folic acid, biopterin. Enzyme is able to reduce dihydrofolate, EC 1.5.1.3 Escherichia coli ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.1.3 FolM
-
 Escherichia coli
1.5.1.50 ydgB
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.3 4.7
-
-
 Escherichia coli
1.5.1.50 4.7
-
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.3 NADPH
-
 Escherichia coli
1.5.1.50 additional information no activity with NADH Escherichia coli
1.5.1.50 NADPH
-
 Escherichia coli

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.5.1.3 0.0059
-
 methotrexate competitive Escherichia coli