EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Moritella profunda |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | methotrexate | IC50: 14.5 nM | Moritella profunda | |
1.5.1.3 | trimethoprim | IC50: 0.0048 mM | Moritella profunda |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0096 | - |
NADPH | pH 7.0, 30°C | Moritella profunda | |
1.5.1.3 | 0.0099 | - |
NADPH | pH 7.0, 5°C | Moritella profunda | |
1.5.1.3 | 0.0123 | - |
NADPH | pH 7.0, 20°C | Moritella profunda | |
1.5.1.3 | 16.4 | - |
7,8-dihydrofolate | pH 7.0, 20°C | Moritella profunda | |
1.5.1.3 | 19.8 | - |
7,8-dihydrofolate | pH 7.0, 30°C | Moritella profunda | |
1.5.1.3 | 21.7 | - |
7,8-dihydrofolate | pH 7.0, 5°C | Moritella profunda |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 18290 | - |
mass spectrometry | Moritella profunda |
1.5.1.3 | 19000 | - |
gel filtration | Moritella profunda |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.1.3 | Moritella profunda | Q70YQ6 | sp. nov. | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.1.3 | - |
Moritella profunda |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.1.3 | 7,8-dihydrofolate + NADPH | - |
Moritella profunda | 5,6,7,8-tetrahydrofolate + NADP+ | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 34 | - |
- |
Moritella profunda |
EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 20 | 50 | 20°C: about 65% of maximal activity, 50°C: about 70% of maximal activity | Moritella profunda |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | additional information | - |
glycerol and 2-mercaptoethanol shift the midpoint of the thermal transition upwards by about 25°C | Moritella profunda |
1.5.1.3 | - |
- |
cold denaturation below | Moritella profunda |
1.5.1.3 | 35 | - |
15 min, stable | Moritella profunda |
1.5.1.3 | 45 | - |
half-life: 25 min | Moritella profunda |
1.5.1.3 | 50 | - |
half-life: 18 min in presence of 0.5 mM 2-mercaptoethanol and 0.5% glycerol | Moritella profunda |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.1.3 | 7 | - |
- |
Moritella profunda |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.1.3 | NADPH | - |
Moritella profunda |
EC Number | IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|---|
1.5.1.3 | 0.0000145 | - |
IC50: 14.5 nM | Moritella profunda | methotrexate | |
1.5.1.3 | 0.0048 | - |
IC50: 0.0048 mM | Moritella profunda | trimethoprim |