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Literature summary extracted from

  • Hashimoto, Y.; Yamamoto, T.; Fujiwara, S.; Takagi, M.; Imanaka, T.
    Extracellular synthesis, specific recognition, and intracellular degradation of cyclomaltodextrins by the hyperthermophilic archaeon Thermococcus sp. strain B1001 (2001), J. Bacteriol., 183, 5050-5057.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.54 gene cgtB, DNA and amino acid sequence determination and analysis, expression in Escherichia coli BL21(DE3) Thermococcus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.54 79000
-
 x * 79000, recombinant enzyme, SDS-PAGE Thermococcus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.54 Thermococcus sp.
-
-
-
3.2.1.54 Thermococcus sp. B1001
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.54 recombinant enzyme from Escherichia coli by heat denaturation Thermococcus sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.54 640
-
 purified recombinant enzyme, substrate gamma-cyclomaltodextrin Thermococcus sp.
3.2.1.54 1080
-
 purified recombinant enzyme, substrate alpha-cyclomaltodextrin Thermococcus sp.
3.2.1.54 1940
-
 purified recombinant enzyme, substrate beta-cyclomaltodextrin Thermococcus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.54 alpha-cyclomaltodextrin + H2O 55% of the activity with beta-cyclomaltodextrin Thermococcus sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 alpha-cyclomaltodextrin + H2O 55% of the activity with beta-cyclomaltodextrin Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
3.2.1.54 beta-cyclomaltodextrin + H2O best substrate Thermococcus sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 beta-cyclomaltodextrin + H2O best substrate Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
3.2.1.54 gamma-cyclomaltodextrin + H2O 33% of the activity with beta-cyclomaltodextrin Thermococcus sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 gamma-cyclomaltodextrin + H2O 33% of the activity with beta-cyclomaltodextrin Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
3.2.1.54 linear maltodextrins + H2O with a small glucose unit, low activity Thermococcus sp. alpha-D-glucose + maltose
-
 ?
3.2.1.54 linear maltodextrins + H2O with a small glucose unit, low activity Thermococcus sp. B1001 alpha-D-glucose + maltose
-
 ?
3.2.1.54 additional information no activity with pullulan and maltose, soluble starch is a poor substrate Thermococcus sp. ?
-
 ?
3.2.1.54 additional information no activity with pullulan and maltose, soluble starch is a poor substrate Thermococcus sp. B1001 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.54 ? x * 79000, recombinant enzyme, SDS-PAGE Thermococcus sp.

Synonyms

EC Number Synonyms Comment Organism
3.2.1.54 CDase
-
 Thermococcus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.54 95
-
 recombinant enzyme Thermococcus sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.54 5.5
-
 recombinant enzyme Thermococcus sp.