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Literature summary extracted from

  • Shearer, N.; Hinsley, A.P.; Van Spanning, R.J.; Spiro, S.
    Anaerobic growth of Paracoccus denitrificans requires cobalamin: characterization of cobK and cobJ genes (1999), J. Bacteriol., 181, 6907-6913.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.54 DNA and amino acid sequence determination and analysis of gene cobK, subcloning of gene cobK for mutant construction in Escherichia coli strain S17-1 Paracoccus denitrificans
2.1.1.131 gene cobJ, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in a pleiotropic mutant strain high has a severe defect in anaerobic growth, the phenotype can be reversed by cobalamin and complemented by expression of genes of the cobalamin biosynthetic pathway, overview Paracoccus denitrificans

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.54 additional information cobK transposon mutagenesis for construction of a pleiotrophic mutant NS52, unable to synthesize cobalamin, and to use ethanolamine as carbon and energy source for aerobic growth, the anaerobic growth of the mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium, phenotype, overview Paracoccus denitrificans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.3.1.54 additional information Paracoccus denitrificans enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium ?
-
 ?
1.3.1.54 additional information Paracoccus denitrificans Pd 1222 enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium ?
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 ?
2.1.1.131 additional information Paracoccus denitrificans Paracoccus denitrificans uses the aerobic pathway for cobalamin biosynthesis ?
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 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B Paracoccus denitrificans enzyme is involved in the aerobic cobalamin biosynthesis S-Adenosyl-L-homocysteine + precorrin-4
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 ?

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.54 Paracoccus denitrificans Q9R9K2
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-
1.3.1.54 Paracoccus denitrificans Pd 1222 Q9R9K2
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-
2.1.1.131 Paracoccus denitrificans Q9R9K1 gene cobJ
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.54 additional information enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium Paracoccus denitrificans ?
-
 ?
1.3.1.54 additional information enzyme is essential for biosynthesis of cobalamin, i.e. vitamin B12, anaerobic growth of the pleiotrophic mutant is affected when either nitrate, nitrite, or nitrous oxide is used as terminal electron acceptor, which is reversible by addition of cobalamine or cobinamide to the minimal medium Paracoccus denitrificans Pd 1222 ?
-
 ?
2.1.1.131 additional information Paracoccus denitrificans uses the aerobic pathway for cobalamin biosynthesis Paracoccus denitrificans ?
-
 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B
-
 Paracoccus denitrificans S-Adenosyl-L-homocysteine + precorrin-4
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 ?
2.1.1.131 S-Adenosyl-L-methionine + precorrin-3B enzyme is involved in the aerobic cobalamin biosynthesis Paracoccus denitrificans S-Adenosyl-L-homocysteine + precorrin-4
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 ?

Synonyms

EC Number Synonyms Comment Organism
1.3.1.54 precorrin-6x reductase
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 Paracoccus denitrificans
2.1.1.131 Precorrin-3B methyltransferase
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 Paracoccus denitrificans