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Literature summary extracted from

  • Nawani, N.N.; Kapadnis, B.P.; Das, A.D.; Rao, A.S.; Mahajan, S.K.
    Purification and characterization of a thermophilic and acidophilic chitinase from Microbispora sp. V2 (2002), J. Appl. Microbiol., 93, 965-975.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Hg2+
-
 Microbispora sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 35000
-
 gel filtration Microbispora sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Microbispora sp.
-
 V2
-
3.2.1.14 Microbispora sp. V2
-
 V2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Microbispora sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 4.6
-
 after Sephadex G-100 purification step Microbispora sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Microbispora sp. N,N',N''-triacetylchitotriose + p-nitrophenol
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N',N''-triacetylchitotriose + H2O
-
 Microbispora sp. V2 N,N',N''-triacetylchitotriose + p-nitrophenol
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
-
 Microbispora sp. N,N'-diacetylchitobiose + p-nitrophenol
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N,N'-diacetylchitobioside + H2O
-
 Microbispora sp. V2 N,N'-diacetylchitobiose + p-nitrophenol
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
-
 Microbispora sp. N-acetyl-D-glucosamine + p-nitrophenol
-
 ?
3.2.1.14 p-nitrophenyl-beta-D-N-acetylglucosaminide + H2O
-
 Microbispora sp. V2 N-acetyl-D-glucosamine + p-nitrophenol
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 60
-
-
 Microbispora sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.14 30
-
 71% activity left after 24 h Microbispora sp.
3.2.1.14 50
-
 45% activity left after 24 h Microbispora sp.
3.2.1.14 60
-
 inactivation after 24 h Microbispora sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 3
-
-
 Microbispora sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 3 11
-
 Microbispora sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.14 3
-
 61% activity left after 1 h Microbispora sp.
3.2.1.14 11
-
 52% activity left after 1 h Microbispora sp.