BRENDA - Enzyme Database show

Aliphatic and enantioselective amidases: from hydrolysis to acyl transfer activity

Fournand, D.; Arnaud, A.; J. Appl. Microbiol. 91, 381-393 (2001)

Data extracted from this reference:

KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.1
-
Benzamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.1
-
butyramide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.1
-
Isobutyramide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.3
-
hexanamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.3
-
nicotinamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
1
-
Propionamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
1.3
-
L-Leucinamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
2
-
L-prolinamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
3
-
Acrylamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
5.4
-
L-Methioninamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
12
-
L-alaninamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
16
-
L-threoninamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
70
-
acetamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.5.1.4
Cyberlindnera jadinii
-
-
-
3.5.1.4
Mycobacterium avium
-
-
-
3.5.1.4
Rhodococcus sp.
-
-
-
3.5.1.4
Rhodococcus sp. R312
-
-
-
3.5.1.4
Sus scrofa
-
-
-
3.5.1.4
Torula cremoris
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
acetamide + H2O
-
655819
Cyberlindnera jadinii
acetate + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
655819
Rhodococcus sp.
acetate + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
655819
Rhodococcus sp. R312
acetate + NH3
-
-
-
?
3.5.1.4
acetamide + hydroxylamine
Ping Pong Bi Bi type mechanism
655819
Rhodococcus sp.
acetylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
acetamide + hydroxylamine
Ping Pong Bi Bi type mechanism
655819
Rhodococcus sp. R312
acetylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
acrylamide + H2O
-
655819
Cyberlindnera jadinii
acrylate + NH3
-
-
-
?
3.5.1.4
acrylamide + hydroxylamine
-
655819
Rhodococcus sp.
acrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
acrylamide + hydroxylamine
-
655819
Rhodococcus sp. R312
acrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
arachidonoyl-ethanolamine + H2O
hydrolysis of anandamide
655819
Sus scrofa
arachidonic acid + ethanolamine
-
-
-
?
3.5.1.4
benzamide + hydroxylamine
-
655819
Rhodococcus sp.
benzoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
benzamide + hydroxylamine
-
655819
Rhodococcus sp. R312
benzoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
655819
Cyberlindnera jadinii
butyrate + NH3
-
-
-
?
3.5.1.4
butyramide + hydroxylamine
-
655819
Rhodococcus sp.
butyrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
butyramide + hydroxylamine
-
655819
Rhodococcus sp. R312
butyrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
hexanamide + H2O
-
655819
Cyberlindnera jadinii
hexanoate + NH3
-
-
-
?
3.5.1.4
hexanamide + hydroxylamine
-
655819
Rhodococcus sp.
hexanoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
isobutyramide + hydroxylamine
-
655819
Rhodococcus sp.
isobutyrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-alaninamide + hydroxylamine
-
655819
Rhodococcus sp.
L-alaninoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-leucinamide + hydroxylamine
-
655819
Rhodococcus sp.
L-leucinoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-methioninamide + hydroxylamine
-
655819
Rhodococcus sp.
L-methioninoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-prolinamide + hydroxylamine
-
655819
Rhodococcus sp.
L-prolinoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-threoninamide + hydroxylamine
-
655819
Rhodococcus sp.
L-threoninoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
additional information
other fatty acid ethanolamides may act as substrate
655819
Sus scrofa
?
-
-
-
-
3.5.1.4
nicotinamide + H2O
absolute specificity
655819
Mycobacterium avium
nicotinic acid + NH3
-
-
-
?
3.5.1.4
nicotinamide + H2O
absolute specificity
655819
Torula cremoris
nicotinic acid + NH3
-
-
-
?
3.5.1.4
nicotinamide + hydroxylamine
-
655819
Rhodococcus sp.
nicotinoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
655819
Cyberlindnera jadinii
propionate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
655819
Rhodococcus sp.
propionate + NH3
-
-
-
?
3.5.1.4
propionamide + hydroxylamine
-
655819
Rhodococcus sp.
propionylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
short-chain amides + hydrazine
-
655819
Rhodococcus sp.
acid hydrazides + NH3
-
-
-
?
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.5.1.4
0.1
-
Benzamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.1
-
butyramide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.1
-
Isobutyramide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.3
-
hexanamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
0.3
-
nicotinamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
1
-
Propionamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
1.3
-
L-Leucinamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
2
-
L-prolinamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
3
-
Acrylamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
5.4
-
L-Methioninamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
12
-
L-alaninamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
16
-
L-threoninamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
3.5.1.4
70
-
acetamide
acyl transfer reaction on hydroxylamine, pH = 7
Rhodococcus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.5.1.4
acetamide + H2O
-
655819
Cyberlindnera jadinii
acetate + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
655819
Rhodococcus sp.
acetate + NH3
-
-
-
?
3.5.1.4
acetamide + H2O
-
655819
Rhodococcus sp. R312
acetate + NH3
-
-
-
?
3.5.1.4
acetamide + hydroxylamine
Ping Pong Bi Bi type mechanism
655819
Rhodococcus sp.
acetylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
acetamide + hydroxylamine
Ping Pong Bi Bi type mechanism
655819
Rhodococcus sp. R312
acetylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
acrylamide + H2O
-
655819
Cyberlindnera jadinii
acrylate + NH3
-
-
-
?
3.5.1.4
acrylamide + hydroxylamine
-
655819
Rhodococcus sp.
acrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
acrylamide + hydroxylamine
-
655819
Rhodococcus sp. R312
acrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
arachidonoyl-ethanolamine + H2O
hydrolysis of anandamide
655819
Sus scrofa
arachidonic acid + ethanolamine
-
-
-
?
3.5.1.4
benzamide + hydroxylamine
-
655819
Rhodococcus sp.
benzoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
benzamide + hydroxylamine
-
655819
Rhodococcus sp. R312
benzoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
butyramide + H2O
-
655819
Cyberlindnera jadinii
butyrate + NH3
-
-
-
?
3.5.1.4
butyramide + hydroxylamine
-
655819
Rhodococcus sp.
butyrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
butyramide + hydroxylamine
-
655819
Rhodococcus sp. R312
butyrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
hexanamide + H2O
-
655819
Cyberlindnera jadinii
hexanoate + NH3
-
-
-
?
3.5.1.4
hexanamide + hydroxylamine
-
655819
Rhodococcus sp.
hexanoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
isobutyramide + hydroxylamine
-
655819
Rhodococcus sp.
isobutyrylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-alaninamide + hydroxylamine
-
655819
Rhodococcus sp.
L-alaninoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-leucinamide + hydroxylamine
-
655819
Rhodococcus sp.
L-leucinoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-methioninamide + hydroxylamine
-
655819
Rhodococcus sp.
L-methioninoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-prolinamide + hydroxylamine
-
655819
Rhodococcus sp.
L-prolinoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
L-threoninamide + hydroxylamine
-
655819
Rhodococcus sp.
L-threoninoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
additional information
other fatty acid ethanolamides may act as substrate
655819
Sus scrofa
?
-
-
-
-
3.5.1.4
nicotinamide + H2O
absolute specificity
655819
Mycobacterium avium
nicotinic acid + NH3
-
-
-
?
3.5.1.4
nicotinamide + H2O
absolute specificity
655819
Torula cremoris
nicotinic acid + NH3
-
-
-
?
3.5.1.4
nicotinamide + hydroxylamine
-
655819
Rhodococcus sp.
nicotinoylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
655819
Cyberlindnera jadinii
propionate + NH3
-
-
-
?
3.5.1.4
propionamide + H2O
-
655819
Rhodococcus sp.
propionate + NH3
-
-
-
?
3.5.1.4
propionamide + hydroxylamine
-
655819
Rhodococcus sp.
propionylhydroxamic acid + NH3
-
-
-
?
3.5.1.4
short-chain amides + hydrazine
-
655819
Rhodococcus sp.
acid hydrazides + NH3
-
-
-
?