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Literature summary extracted from

  • Gomes, R.C.; Semedo, L.T.A.S.; Soares, R.M.A.; Linhares, L.F.; Ulhoa, C.J.; Alviano, C.S.; Coelho, R.R.R.
    Purification of a thermostable endochitinase from Streptomyces RC1071 isolated from a cerrado soil and its antagonism against phytopathogenic fungi (2001), J. Appl. Microbiol., 90, 653-661.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.14 Co2+ slight inhibition Streptomyces sp.
3.2.1.14 EDTA 50 mM, slight inhibition Streptomyces sp.
3.2.1.14 Fe2+ strong inhibition Streptomyces sp.
3.2.1.14 Hg2+ strong inhibition Streptomyces sp.
3.2.1.14 K+ slight inhibition Streptomyces sp.
3.2.1.14 Mg2+ slight inhibition Streptomyces sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information the KM for 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside is 0.02 mmol/l Streptomyces sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 65000
-
 1 * 65000, SDS-PAGE Streptomyces sp.
3.2.1.14 70000
-
 gel filtration Streptomyces sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Streptomyces sp.
-
 RC 1071
-
3.2.1.14 Streptomyces sp. RC 1071
-
 RC 1071
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Streptomyces sp.

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.14 426.3
-
 after affinity chromatography purification step Streptomyces sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.14 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Streptomyces sp. RC 1071 ?
-
 ?
3.2.1.14 chitin + H2O colloidal Streptomyces sp. ?
-
 ?
3.2.1.14 chitin + H2O colloidal Streptomyces sp. RC 1071 ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Streptomyces sp. ?
-
 ?
3.2.1.14 glycol chitin + H2O
-
 Streptomyces sp. RC 1071 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 monomer 1 * 65000, SDS-PAGE Streptomyces sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.14 40
-
-
 Streptomyces sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.14 30 70
-
 Streptomyces sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.14 8
-
-
 Streptomyces sp.

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.14 4 9
-
 Streptomyces sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.14 Streptomyces sp. isoelectric focusing
-
 6