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Literature summary extracted from

  • Shanmugam, V.; Sriram, S.; Babu, S.; Nandakumar, R.; Raguchander, T.; Balasubramanian, P.; Samiyappan, R.
    Purification and characterization of an extracellular alpha-glucosidase protein from Trichoderma viride which degrades a phytotoxin associated with sheath blight disease in rice (2001), J. Appl. Microbiol., 90, 320-329.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.20 biotechnology expression of the enzyme in rice plants can transduce enhanced resistance against sheath blight pathogen Rhizoctonia solani by inactivation of the RS toxin Trichoderma viride

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.4
-
4-nitrophenyl alpha-D-glucopyranoside pH 7.2, 55°C Trichoderma viride

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.2.1.20 extracellular secretion Trichoderma viride
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 110000
-
native PAGE Trichoderma viride

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.20 RS toxin + H2O Trichoderma viride specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth ?
-
?
3.2.1.20 RS toxin + H2O Trichoderma viride TvMNT7 specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Trichoderma viride
-
-
-
3.2.1.20 Trichoderma viride TvMNT7
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20
-
Trichoderma viride

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.20 additional information
-
substrate specificity Trichoderma viride

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Trichoderma viride 4-nitrophenol + alpha-D-glucopyranose
-
?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
Trichoderma viride TvMNT7 4-nitrophenol + alpha-D-glucopyranose
-
?
3.2.1.20 4-nitrophenyl alpha-D-mannopyranoside + H2O
-
Trichoderma viride 4-nitrophenol + alpha-D-mannopyranose
-
?
3.2.1.20 4-nitrophenyl alpha-D-mannopyranoside + H2O
-
Trichoderma viride TvMNT7 4-nitrophenol + alpha-D-mannopyranose
-
?
3.2.1.20 maltose + H2O
-
Trichoderma viride 2 D-glucose
-
?
3.2.1.20 maltose + H2O
-
Trichoderma viride TvMNT7 2 D-glucose
-
?
3.2.1.20 RS toxin + H2O specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth Trichoderma viride ?
-
?
3.2.1.20 RS toxin + H2O host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin, preferred substrate Trichoderma viride ?
-
?
3.2.1.20 RS toxin + H2O specific for the host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin and inhibition of the fungal mycelial growth Trichoderma viride TvMNT7 ?
-
?
3.2.1.20 RS toxin + H2O host-specific Rhizoctonia solani toxin, purified from Rhizoctonia solani-inoculated rice sheath, inactivation of the toxin, preferred substrate Trichoderma viride TvMNT7 ?
-
?
3.2.1.20 starch + H2O
-
Trichoderma viride D-glucose + ?
-
?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 monomer 1 * 110000, SDS-PAGE Trichoderma viride

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 55
-
assay at Trichoderma viride

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 7.2
-
assay at Trichoderma viride

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.20 Trichoderma viride isoelectric focusing
-
4.9