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Literature summary extracted from

  • Rahman, M.; Bhuiyan, S.H.; Nirasawa, S.; Kitaoka, M.; Hayashi, K.
    Characterization of an endo-beta-1,4-glucanase of Thermotoga maritima expressed in Escherichia coli (2002), J. Appl. Glycosci., 49, 487-495.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.4 expression in Escherichia coli Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.4 0.24
-
 p-nitrophenyl-beta-D-cellopentaoside
-
 Thermotoga maritima
3.2.1.4 0.25
-
 p-nitrophenyl-beta-D-cellotetraoside
-
 Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.4 38000
-
 x * 38000, SDS-PAGE Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.4 Thermotoga maritima Q9X273
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.4 recombinant enzyme Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.4 barley glucan + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ?
-
 ?
3.2.1.4 carboxymethylcellulose + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ?
-
 ?
3.2.1.4 lichenan + H2O the enzyme hydrolyzes mixed linked beta-(1-3)(1-4) substrates such as barley glucan and lichenan more strongly than carboxymethylcellulose Thermotoga maritima ?
-
 ?
3.2.1.4 additional information transglycosylation activity with smaller soluble cellooligosaccharides Thermotoga maritima ?
-
 ?
3.2.1.4 p-nitrophenyl-beta-D-cellopentaoside + H2O
-
 Thermotoga maritima ?
-
 ?
3.2.1.4 p-nitrophenyl-beta-D-cellotetraoside + H2O
-
 Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.4 ? x * 38000, SDS-PAGE Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.4 90
-
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.4 85
-
 stable up to Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.4 6.6
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.4 4 9.5 active in the range Thermotoga maritima