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Literature summary extracted from

  • Nihira, T.; Kansarn, S.; Kono, T.; Okada, G.
    Purification and properties of three endo-xylanases from Acremonium cellulolyticus (2001), J. Appl. Glycosci., 48, 45-54.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.8 Ag+ all 3 isozymes Talaromyces pinophilus
3.2.1.8 KMnO4 almost complete inhibition of all 3 isozymes at 1 mM Talaromyces pinophilus
3.2.1.8 PCMB all 3 isozymes Talaromyces pinophilus
3.2.1.8 SDS all 3 isozymes Talaromyces pinophilus
3.2.1.8 Zn2+ all 3 isozymes Talaromyces pinophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.8 additional information
-
 additional information
-
 Talaromyces pinophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.8 25500
-
 x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE Talaromyces pinophilus
3.2.1.8 30000
-
 x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE Talaromyces pinophilus
3.2.1.8 33500
-
 x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE Talaromyces pinophilus

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.8 Talaromyces pinophilus
-
 3 isozymes I, II, and III
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.8 all 3 enzymes Talaromyces pinophilus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.8 74.8
-
 purified isozyme III Talaromyces pinophilus
3.2.1.8 86.1
-
 purified isozyme II Talaromyces pinophilus
3.2.1.8 112.1
-
 purified isozyme I Talaromyces pinophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.8 additional information substrate specificity Talaromyces pinophilus ?
-
 ?
3.2.1.8 xylan + H2O
-
 Talaromyces pinophilus ?
-
 ?
3.2.1.8 xylooligosaccharides + H2O
-
 Talaromyces pinophilus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.8 ? x * 30000, isozyme I, SDS-PAGE, x * 25500, isozyme II, SDS-PAGE, x * 33500, isozyme III, SDS-PAGE Talaromyces pinophilus

Synonyms

EC Number Synonyms Comment Organism
3.2.1.8 xylanase
-
 Talaromyces pinophilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.8 55
-
 all 3 isozymes Talaromyces pinophilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.8 50
-
 isozyme III is stable below Talaromyces pinophilus
3.2.1.8 55
-
 isozymes I and II are stable below Talaromyces pinophilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.8 3.5
-
 isozymes I and III Talaromyces pinophilus
3.2.1.8 3.8
-
 isozyme II Talaromyces pinophilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.8 2.5 9.5 isozyme III, completely stable at 25°C, 2 h Talaromyces pinophilus
3.2.1.8 3 9.5 isozyme II, completely stable at 25°C, 2 h Talaromyces pinophilus
3.2.1.8 3.5 9.5 isozyme I, completely stable at 25°C, 2 h Talaromyces pinophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.8 Talaromyces pinophilus isozyme I
-
 4.9
3.2.1.8 Talaromyces pinophilus isozyme II
-
 4.9
3.2.1.8 Talaromyces pinophilus isozyme III
-
 6