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Literature summary extracted from

  • Valley, M.P.; Tichy, S.E.; Fitzpatrick, P.F.
    Establishing the kinetic competency of the cationic imine intermediate in nitroalkane oxidase (2005), J. Am. Chem. Soc., 127, 2062-2066.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.3.1
-
 Fusarium oxysporum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.3.1 cyanide inactivation of the enzyme by trapping of the electrophilic imine reaction intermediate, formation of a cyanoalkyl intermediate, reaction with the substrate after proton abstraction but before flavin oxidation Fusarium oxysporum
1.7.3.1 Sodium cyanide 2 mM and above, complete inhibition Fusarium oxysporum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.7.3.1 additional information
-
 additional information kinetic competency of the cationic imine enzyme intermediate Fusarium oxysporum

Organism

EC Number Organism UniProt Comment Textmining
1.7.3.1 Fusarium oxysporum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.3.1
-
 Fusarium oxysporum

Reaction

EC Number Reaction Comment Organism Reaction ID
1.7.3.1 ethylnitronate + O2 + FMNH2 = acetaldehyde + nitrite + FMN + H2O mechanism, involves removal of a proton from the nitroalkane, forming a carbanion which adds to the N5 of the flavin, elimination of nitrite from the resulting adduct forms an electrophilic imine intermediate which can be attacked by hydroxide Fusarium oxysporum
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.3.1 1-nitrobutane + O2 + H2O
-
 Fusarium oxysporum butyraldehyde + nitrite + H2O2
-
 ?
1.7.3.1 1-nitrohexane + O2 + H2O
-
 Fusarium oxysporum hexanal + nitrite + H2O2
-
 ?
1.7.3.1 additional information analysis of reaction intermediates formed by mass spectrometry and gel filtration Fusarium oxysporum ?
-
 ?
1.7.3.1 nitroethane + H2O + O2
-
 Fusarium oxysporum acetaldehyde + nitrite + H2O2
-
 ?
1.7.3.1 nitroethane + O2 + H2O
-
 Fusarium oxysporum ethanal + nitrite + H2O2
-
 ?
1.7.3.1 phenylnitromethane + O2 + H2O
-
 Fusarium oxysporum ? + nitrite + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.3.1 NAO
-
 Fusarium oxysporum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.3.1 30
-
 assay at Fusarium oxysporum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.7.3.1 additional information
-
 additional information
-
 Fusarium oxysporum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.3.1 8
-
 assay at Fusarium oxysporum

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.3.1 FAD
-
 Fusarium oxysporum
1.7.3.1 FAD formation of 5-nitroalkyl-FAD intermediates, structure in cyanide inactivated enzyme Fusarium oxysporum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.7.3.1 additional information
-
 additional information inactivation kinetics with different substrates Fusarium oxysporum