Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Koivula, A.; Ruohonen, L.; Wohlfahrt, G.; Reinikainen, T.; Teeri, T.T.; Piens, K.; Claeyssens, M.; Weber, M.; Vasella, A.; Becker, D.; Sinnott, M.L.; Zou, J.Y.; Kleywegt, G.J.; Szardenings, M.; Stahlberg, J.; Jones, T.A.
    The active site of cellobiohydrolase Cel6A from Trichoderma reesei: the roles of aspartic acids D221 and D175 (2002), J. Am. Chem. Soc., 124, 10015-10024.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.91
-
 Trichoderma reesei

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
3.2.1.91
-
 Trichoderma reesei

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.91 D175A mutant retains some activity on longer oligosaccharides Trichoderma reesei
3.2.1.91 D221A almost complete loss of activity Trichoderma reesei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.91 1.3
-
 cellopentaose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 1.4
-
 cellopentaose pH 5, 27°C, D175A mutant Trichoderma reesei
3.2.1.91 2.6
-
 cellotetraose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 4
-
 cellotetraose pH 5, 27°C, D175A mutant Trichoderma reesei
3.2.1.91 14
-
 cellohexaose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 17
-
 cellotriose pH 5, 27°C, wild-type enzyme Trichoderma reesei

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.91 Trichoderma reesei P07987
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.91 cellohexaose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.91 cellopentaose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.91 cellotetraose + H2O
-
 Trichoderma reesei ?
-
 ?
3.2.1.91 cellotriose + H2O
-
 Trichoderma reesei ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.91 0.0003
-
 cellotriose pH 5, 27°C, D175A mutant Trichoderma reesei
3.2.1.91 0.0013
-
 cellotetraose pH 5, 27°C, D175A mutant Trichoderma reesei
3.2.1.91 0.02
-
 cellohexaose pH 5, 27°C, D175A mutant Trichoderma reesei
3.2.1.91 0.025
-
 cellopentaose pH 5, 27°C, D175A mutant Trichoderma reesei
3.2.1.91 0.06
-
 cellotriose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 1.1
-
 cellopentaose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 4.1
-
 cellotetraose pH 5, 27°C, wild-type enzyme Trichoderma reesei
3.2.1.91 14
-
 cellohexaose pH 5, 27°C, wild-type enzyme Trichoderma reesei