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Literature summary extracted from
- Beta-glucosidase from the grape native yeast Debaryomyces vanrijiae: purification, characterization, and its effect on monoterpene content of a Muscat grape juice (2003), J. Agric. Food Chem., 51, 1453-1459.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | Cu2+ | 48% inhibition | Schwanniomyces vanrijiae |  |
| 3.2.1.21 | D-glucono-delta-lactone | - |
Schwanniomyces vanrijiae | |
| 3.2.1.21 | D-glucose | - |
Schwanniomyces vanrijiae | |
| 3.2.1.21 | ethanol | 10-15%, 20-40% inhibition, complete inhibition above 30% | Schwanniomyces vanrijiae | |
| 3.2.1.21 | Sodium dodecyl sulfate | 0.4%, complete inhibition | Schwanniomyces vanrijiae | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.77 | - |
p-nitro-phenyl-beta-D-glucopyranoside | 40°C, pH 5.0 | Schwanniomyces vanrijiae | |
| 3.2.1.21 | 1.07 | - |
geranyl-beta-D-glucopyranoside | 40°C, pH 5.0 | Schwanniomyces vanrijiae | |
| 3.2.1.21 | 57.9 | - |
cellobiose | 40°C, pH 5.0 | Schwanniomyces vanrijiae | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 100000 | - |
x * 100000, SDS-PAGE | Schwanniomyces vanrijiae |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Schwanniomyces vanrijiae | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | glycoprotein | - |
Schwanniomyces vanrijiae |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | ultrafiltration, Ultrogel, DEAE-Sepharose, chromatofocusing | Schwanniomyces vanrijiae |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 1278 | - |
- |
Schwanniomyces vanrijiae |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | benzyl-beta-D-glucopyranoside + H2O | - |
Schwanniomyces vanrijiae | benzyl alcohol + beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Schwanniomyces vanrijiae | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | geranyl-beta-D-glucopyranoside + H2O | - |
Schwanniomyces vanrijiae | geraniol + beta-D-glucose | - |
? | |
| 3.2.1.21 | linalool-beta-D-glucopyranoside + H2O | - |
Schwanniomyces vanrijiae | linalool + beta-D-glucose | - |
? | |
| 3.2.1.21 | nerol-beta-D-glucopyranoside + H2O | - |
Schwanniomyces vanrijiae | nerol + beta-D-glucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Schwanniomyces vanrijiae | p-nitrophenol + beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 100000, SDS-PAGE | Schwanniomyces vanrijiae |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | DV-BG | - |
Schwanniomyces vanrijiae |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | - |
- |
Schwanniomyces vanrijiae |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 20 | 60 | approx. 40% of maximal activity at 20°C, approx. 205 of maximal activity at 60°C | Schwanniomyces vanrijiae |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 45 | - |
no loss of activity after 1 h | Schwanniomyces vanrijiae |
| 3.2.1.21 | 50 | - |
approx. 40% loss of activity after 1 h | Schwanniomyces vanrijiae |
| 3.2.1.21 | 60 | - |
approx. 60% loss of activity after 1 h | Schwanniomyces vanrijiae |
| 3.2.1.21 | 70 | - |
complete loss of activity after 1 h | Schwanniomyces vanrijiae |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
- |
Schwanniomyces vanrijiae |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3.15 | - |
approx. 80% loss of activity after 24 h | Schwanniomyces vanrijiae |
| 3.2.1.21 | 4 | 5.5 | approx. 50% loss of activity after 24 h | Schwanniomyces vanrijiae |
| 3.2.1.21 | 7 | - |
approx. 40% loss of activity after 24 h | Schwanniomyces vanrijiae |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 5 | - |
D-glucono-delta-lactone | 40°C, pH 5.0 | Schwanniomyces vanrijiae | |
| 3.2.1.21 | 439 | - |
D-glucose | 40°C, pH 5.0 | Schwanniomyces vanrijiae | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.2.1.21 | Schwanniomyces vanrijiae | chromatofocusing, narrow-range isoelectric focusing | - |
3 |
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