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Literature summary extracted from
- Purification and characterization of a beta-glucosidase from Citrus sinensis var. Valencia fruit tissue (2001), J. Agric. Food Chem., 49, 4457-4462.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | beta-D-glucose | 5 mM, approx. 50% inhibition, 100 mM, almost complete inhibition | Citrus sinensis |  |
| 3.2.1.21 | gluconolactone | 10 mM, 91% inhibition, 1 mM, 70% inhibition | Citrus sinensis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.12 | - |
p-nitrophenyl-beta-D-glucopyranose | 40°C, pH 5.0 | Citrus sinensis | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 64000 | - |
x * 64000, SDS-PAGE | Citrus sinensis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | arbutin + H2O | Citrus sinensis | 104% of activity with p-nitrophenyl-beta-D-glucopyranoside | 4-hydroxyphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | Citrus sinensis | 609% of activity with p-nitrophenyl-beta-D-glucopyranoside | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiobiose + H2O | Citrus sinensis | 483% of activity with p-nitrophenyl-beta-D-glucopyranoside | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | hesperetin 7-O-beta-glucopyranoside + H2O | Citrus sinensis | 199% of activity with p-nitrophenyl-beta-D-glucopyranoside | hesperetin + beta-D-glucose | - |
? | |
| 3.2.1.21 | hesperitin-dihydrochalcon 7-O-beta-D-glucopyranoside + H2O | Citrus sinensis | 91% of activity with p-nitrophenyl-beta-D-glucopyranoside | hesperitin-dihydrochalcon + beta-D-glucose | - |
? | |
| 3.2.1.21 | laminaribiose + H2O | Citrus sinensis | 519% of activity with p-nitrophenyl-beta-D-glucopyranoside | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | mandelonitrile-beta-D-glucopyranoside + H2O | Citrus sinensis | trivial name prunasin, 309% of activity with p-nitrophenyl-beta-D-glucopyranoside | mandelonitrile + beta-D-glucose | - |
? | |
| 3.2.1.21 | salicin + H2O | Citrus sinensis | 390% of activity with p-nitrophenyl-beta-D-glucopyranoside | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | sinigrin + H2O | Citrus sinensis | 325% of activity with p-nitrophenyl-beta-D-glucopyranoside | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | sophorose + H2O | Citrus sinensis | 430% of activity with p-nitrophenyl-beta-D-glucopyranoside | 2 beta-D-glucose | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Citrus sinensis | - |
var. Valencia | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | DEAE-Sephacel, CM-Sepharose, Superdex, Toyopearl | Citrus sinensis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | fruit | - |
Citrus sinensis | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 1.13 | - |
- |
Citrus sinensis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl alpha-D-glucopyranoside + H2O | 7% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | 4-nitrophenol + D-glucose | - |
? | |
| 3.2.1.21 | arbutin + H2O | 104% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | 4-hydroxyphenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | 609% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiobiose + H2O | 483% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | hesperetin 7-O-beta-glucopyranoside + H2O | 199% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | hesperetin + beta-D-glucose | - |
? | |
| 3.2.1.21 | hesperitin-dihydrochalcon 7-O-beta-D-glucopyranoside + H2O | 91% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | hesperitin-dihydrochalcon + beta-D-glucose | - |
? | |
| 3.2.1.21 | laminaribiose + H2O | 519% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | 2 beta-D-glucose | - |
? | |
| 3.2.1.21 | mandelonitrile-beta-D-glucopyranoside + H2O | trivial name prunasin, 309% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | mandelonitrile + beta-D-glucose | - |
? | |
| 3.2.1.21 | naringenin-7-beta-glucopyranoside + H2O | 138% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | naringenin + beta-D-glucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-alpha-D-arabinofuranoside + H2O | 3.7% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | p-nitrophenol + alpha-D-arabinose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-alpha-D-mannopyranoside + H2O | 1.1% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | p-nitrophenol + alpha-D-mannose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-alpha-D-rhamnopyranoside + H2O | 1.9% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | p-nitrophenol + alpha-D-rhamnose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-fucopyranoside + H2O | 13.5% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | p-nitrophenol + beta-D-fucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Citrus sinensis | p-nitrophenol + beta-D-glucose | - |
? | |
| 3.2.1.21 | p-nitrophenyl-beta-D-xylopyranoside + H2O | 1.9% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | p-nitrophenol + beta-D-xylose | - |
? | |
| 3.2.1.21 | salicin + H2O | 390% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | sinigrin + H2O | 325% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | beta-D-glucose + ? | - |
? | |
| 3.2.1.21 | sophorose + H2O | 430% of activity with p-nitrophenyl-beta-D-glucopyranoside | Citrus sinensis | 2 beta-D-glucose | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.2.1.21 | ? | x * 64000, SDS-PAGE | Citrus sinensis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 40 | 45 | - |
Citrus sinensis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 30 | 60 | approx. 70% of maximal activity at 30°C, approx. 13% of maximal activity at 60°C | Citrus sinensis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4.5 | 5.5 | - |
Citrus sinensis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 3 | 7 | approx. 20% of maximal activity at pH 3.5, approx. 40% of maximal activity at pH 7.0 | Citrus sinensis |
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