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Literature summary extracted from

  • McCarthy, T.; Hanniffy, O.; Savage, A.V.; Tuohy, M.G.
    Catalytic properties and mode of action of three endo-beta-glucanases from Talaromyces emersonii on soluble beta-1,4- and beta-1,3;1,4-linked glucans (2003), Int. J. Biol. Macromol., 33, 141-148.
    View publication on PubMed

Application

EC Number Application Comment Organism
3.2.1.6 nutrition endo-beta-D-glucanases EG VI and EG VII are ideal candidates for tailored modification of macromolecular beta-glucan in industrial applications Rasamsonia emersonii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.6 additional information
-
 additional information pH 5, 50°C, EG VI: Km for barley 1,3-1,4-beta-D-glucan is 28.6 mg/ml, Km for lichenan is 58.8 mg/ml, Km for carboxymethylcellulose is 76.9 mg/ml, EG VII: Km for barley 1,3-1,4-beta-D-glucan is 9.1 mg/ml, Km for carboxymethylcellulose is 62.5 mg/ml Rasamsonia emersonii

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.6 Rasamsonia emersonii
-
 two endo-beta-D-glucanases EG VI and EG VII
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3.2.1.6 Rasamsonia emersonii CBS 814.70
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 two endo-beta-D-glucanases EG VI and EG VII
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.6 EG VI and EG VII Rasamsonia emersonii

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.6 (Glcbeta(1-3))n + H2O = laminaripentaose + (Glcbeta(1-3))n-5 substrates include laminarin, lichenin, and cereal D-glucans, different from EC 3.2.1.52 beta-L-N-acetylhexosaminidase Rasamsonia emersonii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.6 1,3-1,4-beta-D-glucan + H2O soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan Rasamsonia emersonii cellooligosaccharide spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation ?
3.2.1.6 1,3-1,4-beta-D-glucan + H2O soluble, from barley, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, highest catalytic efficiency with barley 1,3-1,4-beta-D-glucan Rasamsonia emersonii CBS 814.70 cellooligosaccharide spectrum of oligosaccharide products by EG VI and EG VII including linear beta-1,4-gluco and mixed-link oligosaccharides, relative distribution of the cellooligosaccharides DP 1-5 after 24 h incubation ?
3.2.1.6 carboxymethylcellulose + H2O soluble, 1,4-beta-D-glucan, endo-acting enzyme Rasamsonia emersonii cellobiose + cellotriose + glucose EG VI releases mainly cellobiose and almost equimolar amounts of cellotriose and glucose, EG VII yields mainly cellobiose and glucose as final reaction products ?
3.2.1.6 carboxymethylcellulose + H2O soluble, 1,4-beta-D-glucan, endo-acting enzyme Rasamsonia emersonii CBS 814.70 cellobiose + cellotriose + glucose EG VI releases mainly cellobiose and almost equimolar amounts of cellotriose and glucose, EG VII yields mainly cellobiose and glucose as final reaction products ?
3.2.1.6 laminaran + H2O from Laminaria digitata, 1,3-beta-glucan Rasamsonia emersonii ?
-
 ?
3.2.1.6 laminaran + H2O from Laminaria digitata, 1,3-beta-glucan Rasamsonia emersonii CBS 814.70 ?
-
 ?
3.2.1.6 lichenan + H2O from Cetraria islandica, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, rapid hydrolysis Rasamsonia emersonii cellooligosaccharide spectrum of oligosaccharide products by EG VI and EG VII ?
3.2.1.6 lichenan + H2O from Cetraria islandica, endo-acting enzyme, preference for mixed-link 1,3-1,4-beta-D-glucan substrates, rapid hydrolysis Rasamsonia emersonii CBS 814.70 cellooligosaccharide spectrum of oligosaccharide products by EG VI and EG VII ?
3.2.1.6 additional information one active site per enzyme molecule Rasamsonia emersonii ?
-
 ?
3.2.1.6 additional information one active site per enzyme molecule Rasamsonia emersonii CBS 814.70 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.2.1.6 beta-1,3-1,4-glucanase
-
 Rasamsonia emersonii
3.2.1.6 endo-1,3-1,4-beta-D-glucanase
-
 Rasamsonia emersonii
3.2.1.6 endo-1,3-beta-D-glucanase
-
 Rasamsonia emersonii
3.2.1.6 laminaranase
-
 Rasamsonia emersonii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.6 50
-
 assay at Rasamsonia emersonii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.6 additional information
-
 EG VI and EG VII are thermostable Rasamsonia emersonii

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.6 114.8
-
 1,3-1,4-beta-D-glucan pH 5, 50°C, soluble, from barley, EG VII Rasamsonia emersonii
3.2.1.6 142.6
-
 carboxymethylcellulose pH 5, 50°C, EG VII Rasamsonia emersonii
3.2.1.6 174.7
-
 lichenan pH 5, 50°C, EG VI Rasamsonia emersonii
3.2.1.6 281.2
-
 1,3-1,4-beta-D-glucan pH 5, 50°C, soluble, from barley, EG VI Rasamsonia emersonii
3.2.1.6 632.8
-
 carboxymethylcellulose pH 5, 50°C, EG VI Rasamsonia emersonii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.6 5
-
 assay at Rasamsonia emersonii