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Literature summary extracted from

  • Oezer, N.; Bilgi, C.; Hamdi Ogus, I.
    Dog liver glucose-6-phosphate dehydrogenase: purification and kinetic properties (2002), Int. J. Biochem. Cell Biol., 34, 253-262.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.49 NADPH has a regulatory role in pentaose phosphate pathway Canis lupus familiaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 additional information
-
 additional information kinetics and thermodynamics, rapid equilibrium random bi bi kinetic model Canis lupus familiaris
1.1.1.49 0.01
-
 NADP+ pH 7.4, 37°C, with D-glucose 6-phosphate or 2-deoxy-D-glucose 6-phosphate Canis lupus familiaris
1.1.1.49 0.122
-
 D-glucose 6-phosphate pH 7.4, 37°C, with NADP+ or deamino-NADP+ Canis lupus familiaris
1.1.1.49 2 3 deamino-NADP+ pH 7.4, 37°C, with D-glucose 6-phosphate Canis lupus familiaris
1.1.1.49 24
-
 2-deoxy-D-glucose 6-phosphate pH 7.4, 37°C, with NADP+ Canis lupus familiaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.49 Mg2+ required, can substitute for Mn2+ Canis lupus familiaris
1.1.1.49 Mn2+ required, can substitute for Mg2+ Canis lupus familiaris
1.1.1.49 additional information high ionic strength inactivates the enzyme Canis lupus familiaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.49 additional information
-
 native PAGE Canis lupus familiaris
1.1.1.49 52500
-
 2 * 52500, SDS-PAGE Canis lupus familiaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NAD(P)+ Canis lupus familiaris key enzyme catalyzing the first step of the pentose phosphate pathway generating NADPH for anabolic metabolsim pathways and protection systems in the liver D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Canis lupus familiaris
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 2000fold by 2times 2',5'-ADP affinity chromatography and gel filtration Canis lupus familiaris

Reaction

EC Number Reaction Comment Organism Reaction ID
1.1.1.49 D-glucose 6-phosphate + NADP+ = 6-phospho-D-glucono-1,5-lactone + NADPH + H+ rapid equilibrium random bi bi kinetic mechanism Canis lupus familiaris
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.49 liver
-
 Canis lupus familiaris
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.49 130
-
 purified enzyme Canis lupus familiaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 2-deoxy-D-glucose 6-phosphate + NADP+
-
 Canis lupus familiaris 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NADPH
-
 ?
1.1.1.49 D-glucose 6-phosphate + deamino-NADP+
-
 Canis lupus familiaris D-glucono-1,5-lactone 6-phosphate + deamino-NADPH
-
 ?
1.1.1.49 D-glucose 6-phosphate + NAD(P)+ key enzyme catalyzing the first step of the pentose phosphate pathway generating NADPH for anabolic metabolsim pathways and protection systems in the liver Canis lupus familiaris D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
 ?
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Canis lupus familiaris D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.49 dimer 2 * 52500, SDS-PAGE Canis lupus familiaris
1.1.1.49 More low pH value and low ionic strength cause aggregation of the enzyme in active oligomeric forms, e.g. as tetramer or hexamer Canis lupus familiaris

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G6PD
-
 Canis lupus familiaris
1.1.1.49 glucose-6-phosphate dehydrogenase
-
 Canis lupus familiaris

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.49 37
-
 assay at Canis lupus familiaris

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 7.8
-
-
 Canis lupus familiaris

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.49 additional information
-
 high pH value inactivates the enzyme Canis lupus familiaris

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 deamino-NADP+ not as effective as NADP+ Canis lupus familiaris
1.1.1.49 NADP+
-
 Canis lupus familiaris

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.49 0.012
-
 NADPH pH 7.4, 37°C Canis lupus familiaris