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Literature summary extracted from

  • Arakane, Y.; Zhu, Q.; Matsumiya, M.; Muthukrishnan, S.; Kramer, K.J.
    Properties of catalytic, linker and chitin-binding domains of insect chitinase (2003), Insect Biochem. Mol. Biol., 33, 631-648.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.14 expression in Hi-5 insect cells using baculovirus vectors Manduca sexta

Protein Variants

EC Number Protein Variants Comment Organism
3.2.1.14 DELTA1-376 ChiLCH, with a C-terminal His tag Manduca sexta
3.2.1.14 DELTA1-477 ChiCH, with a C-terminal His tag Manduca sexta
3.2.1.14 DELTA1-79 Chi80-535, enzymatically inactive Manduca sexta
3.2.1.14 DELTA377-535 Chi376, no secretion into the medium Manduca sexta
3.2.1.14 DELTA387-477 Chi386(MCBD) Manduca sexta
3.2.1.14 DELTA387-535 Chi386 Manduca sexta
3.2.1.14 DELTA397-535 Chi396 Manduca sexta
3.2.1.14 DELTA408-535 Chi407 Manduca sexta
3.2.1.14 DELTA478-535 Chi477 Manduca sexta
3.2.1.14 additional information Chi386(MCBD)2: Chi386 protein fused with doubbled amino acid residues 478-535. Chi386(RCBD): Chi386 protein fused with amino acid residues 19-68 of a rice class I chitinase. ChiLH: linker region consisting of amino acid residues 377-477 with a C-terminal His tag, no secretion into the medium Manduca sexta

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.14 additional information
-
 additional information the KM-values with chitin as substrate are for wild-type: 4.29 mg/ml, Chi477: 9.96 mg/ml, Chi396: 20 mg/ml, Chi386: 29 mg/ml, Chi386(MCBD): 4.98 mg/ml, Chi386(MCBD)2: 4.67 mg/ml and Chi386(RCBD): 3.18 mg/ml Manduca sexta
3.2.1.14 0.29
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD) Manduca sexta
3.2.1.14 0.31
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD)2 Manduca sexta
3.2.1.14 0.48
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(RCBD) Manduca sexta
3.2.1.14 0.51
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi477 Manduca sexta
3.2.1.14 0.64
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi396 Manduca sexta
3.2.1.14 0.93
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386 Manduca sexta
3.2.1.14 1.13
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, wild-type Manduca sexta

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.14 79000
-
 x * 79000, SDS-PAGE Manduca sexta

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.14 Manduca sexta
-
 tobacco hornworm
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
3.2.1.14 glycoprotein glycosylated with mannose and N-acetylglucosamine as most abundant sugars, glucose and galactose Manduca sexta

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.14
-
 Manduca sexta

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.14 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside + H2O
-
 Manduca sexta ?
-
 ?
3.2.1.14 chitin + H2O
-
 Manduca sexta ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.14 ? x * 79000, SDS-PAGE Manduca sexta

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.14 0.14
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD) Manduca sexta
3.2.1.14 0.17
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(MCBD)2 Manduca sexta
3.2.1.14 0.22
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386(RCBD) Manduca sexta
3.2.1.14 0.27
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi396 Manduca sexta
3.2.1.14 0.31
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi477 Manduca sexta
3.2.1.14 0.37
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, Chi386 Manduca sexta
3.2.1.14 0.76
-
 4-methylumbelliferyl beta-N,N',N''-triacetylchitotrioside 37°C, pH 6, wild-type Manduca sexta