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Literature summary extracted from

  • Ferreira, A.H.; Terra, W.R.; Ferreira, C.
    Characterization of a beta-glycosidase highly active on disaccharides and of a beta-galactosidase from Tenebrio molitor midgut lumen (2003), Insect Biochem. Mol. Biol., 33, 253-265.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.23 gamma-galactonolactone
-
 Tenebrio molitor
3.2.1.23 lactose
-
 Tenebrio molitor
3.2.1.23 octyl-beta-thio-galactoside
-
 Tenebrio molitor
3.2.1.23 p-nitrophenyl beta-D-fucoside
-
 Tenebrio molitor
3.2.1.23 p-nitrophenyl beta-D-galactopyranoside
-
 Tenebrio molitor

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.23 0.14
-
 4-methylumbelliferyl beta-D-galactoside
-
 Tenebrio molitor
3.2.1.23 21
-
 p-nitrophenyl beta-D-fucoside
-
 Tenebrio molitor
3.2.1.23 250
-
 lactose
-
 Tenebrio molitor

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.23 30000
-
 2 * 30000, SDS-PAGE Tenebrio molitor
3.2.1.23 64000
-
 gel filtration Tenebrio molitor

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.23 Tenebrio molitor
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.23
-
 Tenebrio molitor

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.2.1.23 larva midgut Tenebrio molitor
-
3.2.1.23 midgut lumen Tenebrio molitor
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.23 13.7
-
-
 Tenebrio molitor

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.23 4-methylumbelliferyl beta-D-galactoside + H2O
-
 Tenebrio molitor 4-methylumbelliferone + beta-D-galactose
-
 ?
3.2.1.23 lactose + H2O
-
 Tenebrio molitor D-glucose + D-galactose
-
 ?
3.2.1.23 p-nitrophenyl beta-D-fucoside + H2O
-
 Tenebrio molitor p-nitrophenol + beta-D-fucose
-
 ?
3.2.1.23 p-nitrophenyl beta-D-galactopyranoside + H2O
-
 Tenebrio molitor p-nitrophenol + beta-D-galactose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.23 dimer 2 * 30000, SDS-PAGE Tenebrio molitor

Synonyms

EC Number Synonyms Comment Organism
3.2.1.23 betaGly4
-
 Tenebrio molitor

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.23 45
-
 pH 5.5, more than 80% loss of activity after 430 min Tenebrio molitor

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.23 1.2
-
 lactose
-
 Tenebrio molitor
3.2.1.23 1.7
-
 4-methylumbelliferyl beta-D-galactoside
-
 Tenebrio molitor
3.2.1.23 2.4
-
 p-nitrophenyl beta-D-fucoside
-
 Tenebrio molitor

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.23 0.0081
-
 gamma-galactonolactone hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor
3.2.1.23 0.02
-
 gamma-galactonolactone hydrolysis of p-nitrophenyl beta-D-galactoside Tenebrio molitor
3.2.1.23 0.34
-
 octyl-beta-thio-galactoside hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor
3.2.1.23 0.7
-
 p-nitrophenyl beta-D-galactopyranoside hydrolysis of 4-methylumbellipheryl beta-D-galactoside Tenebrio molitor
3.2.1.23 1
-
 octyl-beta-thio-galactoside hydrolysis of p-nitrophenyl beta-D-galactoside Tenebrio molitor
3.2.1.23 4.3
-
 p-nitrophenyl beta-D-fucoside hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor
3.2.1.23 260
-
 lactose hydrolysis of 4-methylumbelliferyl beta-D-galactoside Tenebrio molitor