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Literature summary extracted from

  • Eneyskaya, E.V.; Kulminskaya, A.A.; Kalkkinen, N.; Nifantiev, N.E.; Arbatskii, N.P.; Saenko, A.I.; Chepurnaya, O.V.; Arutyunyan, A.V.; Shabalin, K.A.; Neustroev, K.N.
    An alpha-L-fucosidase from Thermus sp. with unusually broad specificity (2001), Glycoconj. J., 18, 827-834.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 alpha-L-fucose competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis Thermus sp.
3.2.1.51 fucose
-
 Thermus sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.2
-
 p-nitrophenyl alpha-L-fucopyranoside pH 6.5, 37°C Thermus sp.
3.2.1.51 3
-
 alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc pH 6.5, 37°C Thermus sp.
3.2.1.51 7
-
 alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me pH 6.5, 37°C Thermus sp.
3.2.1.51 9
-
 beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc pH 6.5, 37°C Thermus sp.
3.2.1.51 16
-
 methyl alpha-L-fucopyranoside pH 6.5, 37°C Thermus sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 61000
-
 4 * 61000, SDS-PAGE Thermus sp.
3.2.1.51 240000
-
 gel filtration Thermus sp.

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Thermus sp.
-
-
-
3.2.1.51 Thermus sp. Y5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Thermus sp.

Reaction

EC Number Reaction Comment Organism Reaction ID
3.2.1.51 an alpha-L-fucoside + H2O = L-fucose + an alcohol the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides Thermus sp.
a

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 4°C, enzyme freeze dried from aqueous solution containing 3 mM DTT and 5-20 mg/ml bovine serum albumin retains full activity for at least one year Thermus sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
 Thermus sp. alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
 ?
3.2.1.51 alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
 Thermus sp. Y5 alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
 ?
3.2.1.51 alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
 Thermus sp. alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
 ?
3.2.1.51 alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
 Thermus sp. Y5 alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
 ?
3.2.1.51 beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
 Thermus sp. alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
 ?
3.2.1.51 beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
 Thermus sp. Y5 alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
 ?
3.2.1.51 methyl alpha-L-fucopyranoside + H2O or methanol or ethanol as acceptor Thermus sp. methanol + alpha-L-fucose
-
 ?
3.2.1.51 methyl alpha-L-fucopyranoside + H2O or methanol or ethanol as acceptor Thermus sp. Y5 methanol + alpha-L-fucose
-
 ?
3.2.1.51 additional information not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside Thermus sp. ?
-
 ?
3.2.1.51 additional information not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside Thermus sp. Y5 ?
-
 ?
3.2.1.51 p-nitrophenyl alpha-L-fucopyranoside + H2O or methanol or ethanol as acceptor Thermus sp. p-nitrophenol + alpha-L-fucopyranose
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 tetramer 4 * 61000, SDS-PAGE Thermus sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 50
-
 optimal temperatures for hydrolysis Thermus sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 37
-
 the enzyme is stable overnight in the pH range from 5.5 to 7.5 Thermus sp.
3.2.1.51 45
-
 thermal inactivation at temperatures above 45°C Thermus sp.
3.2.1.51 60
-
 the activity decreases rapidly at temperatures above 60°C Thermus sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 5.5 7.5 the enzyme is stable overnight at 37°C Thermus sp.

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 0.15
-
 fucose pH 6.5, 37°C Thermus sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.51 Thermus sp.
-
-
 4.2