BRENDA - Enzyme Database

An alpha-L-fucosidase from Thermus sp. with unusually broad specificity

Eneyskaya, E.V.; Kulminskaya, A.A.; Kalkkinen, N.; Nifantiev, N.E.; Arbatskii, N.P.; Saenko, A.I.; Chepurnaya, O.V.; Arutyunyan, A.V.; Shabalin, K.A.; Neustroev, K.N.; Glycoconj. J. 18, 827-834 (2001)

Data extracted from this reference:

Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis
Thermus sp.
3.2.1.51
fucose
-
Thermus sp.
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.2
-
p-nitrophenyl alpha-L-fucopyranoside
pH 6.5, 37°C
Thermus sp.
3.2.1.51
3
-
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc
pH 6.5, 37°C
Thermus sp.
3.2.1.51
7
-
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
pH 6.5, 37°C
Thermus sp.
3.2.1.51
9
-
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc
pH 6.5, 37°C
Thermus sp.
3.2.1.51
16
-
methyl alpha-L-fucopyranoside
pH 6.5, 37°C
Thermus sp.
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
61000
-
4 * 61000, SDS-PAGE
Thermus sp.
3.2.1.51
240000
-
gel filtration
Thermus sp.
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
3.2.1.51
Thermus sp.
-
-
-
3.2.1.51
Thermus sp. Y5
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
3.2.1.51
-
Thermus sp.
Reaction
EC Number
Reaction
Commentary
Organism
3.2.1.51
an alpha-L-fucoside + H2O = L-fucose + an alcohol
the enzyme cleaves alpha1-6-, alpha1-3-, alpha1-4- and alpha1-2-O-fucolsyl bonds in fucosylated oligosaccharides
Thermus sp.
Storage Stability
EC Number
Storage Stability
Organism
3.2.1.51
4°C, enzyme freeze dried from aqueous solution containing 3 mM DTT and 5-20 mg/ml bovine serum albumin retains full activity for at least one year
Thermus sp.
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
655691
Thermus sp.
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
655691
Thermus sp. Y5
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
655691
Thermus sp.
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
655691
Thermus sp. Y5
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
?
3.2.1.51
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
655691
Thermus sp.
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
?
3.2.1.51
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
655691
Thermus sp. Y5
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
?
3.2.1.51
methyl alpha-L-fucopyranoside + H2O
or methanol or ethanol as acceptor
655691
Thermus sp.
methanol + alpha-L-fucose
-
-
-
?
3.2.1.51
methyl alpha-L-fucopyranoside + H2O
or methanol or ethanol as acceptor
655691
Thermus sp. Y5
methanol + alpha-L-fucose
-
-
-
?
3.2.1.51
additional information
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
655691
Thermus sp.
?
-
-
-
-
3.2.1.51
additional information
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
655691
Thermus sp. Y5
?
-
-
-
-
3.2.1.51
p-nitrophenyl alpha-L-fucopyranoside + H2O
or methanol or ethanol as acceptor
655691
Thermus sp.
p-nitrophenol + alpha-L-fucopyranose
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 61000, SDS-PAGE
Thermus sp.
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
optimal temperatures for hydrolysis
Thermus sp.
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
37
-
the enzyme is stable overnight in the pH range from 5.5 to 7.5
Thermus sp.
3.2.1.51
45
-
thermal inactivation at temperatures above 45°C
Thermus sp.
3.2.1.51
60
-
the activity decreases rapidly at temperatures above 60°C
Thermus sp.
pH Stability
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
5.5
7.5
the enzyme is stable overnight at 37°C
Thermus sp.
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.15
-
fucose
pH 6.5, 37°C
Thermus sp.
pI Value
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.51
Thermus sp.
-
-
4.2
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
3.2.1.51
alpha-L-fucose
competivite inhibition of p-nitrophenyl alpha-L-fucopyranoside hydrolysis
Thermus sp.
3.2.1.51
fucose
-
Thermus sp.
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
3.2.1.51
0.15
-
fucose
pH 6.5, 37°C
Thermus sp.
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
3.2.1.51
0.2
-
p-nitrophenyl alpha-L-fucopyranoside
pH 6.5, 37°C
Thermus sp.
3.2.1.51
3
-
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc
pH 6.5, 37°C
Thermus sp.
3.2.1.51
7
-
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
pH 6.5, 37°C
Thermus sp.
3.2.1.51
9
-
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc
pH 6.5, 37°C
Thermus sp.
3.2.1.51
16
-
methyl alpha-L-fucopyranoside
pH 6.5, 37°C
Thermus sp.
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
3.2.1.51
61000
-
4 * 61000, SDS-PAGE
Thermus sp.
3.2.1.51
240000
-
gel filtration
Thermus sp.
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
3.2.1.51
-
Thermus sp.
Storage Stability (protein specific)
EC Number
Storage Stability
Organism
3.2.1.51
4°C, enzyme freeze dried from aqueous solution containing 3 mM DTT and 5-20 mg/ml bovine serum albumin retains full activity for at least one year
Thermus sp.
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
655691
Thermus sp.
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-3)-D-GalNAc + H2O
-
655691
Thermus sp. Y5
alpha-L-fucose + beta-D-Gal-(1-3)-D-GalNAc
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
655691
Thermus sp.
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
?
3.2.1.51
alpha-L-Fuc-(1-2)-beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me + H2O
-
655691
Thermus sp. Y5
alpha-L-fucose + beta-D-Gal-(1-4)-beta-D-GlcNAc-1-Me
-
-
-
?
3.2.1.51
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
655691
Thermus sp.
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
?
3.2.1.51
beta-D-GlcNAc-(1-4)-(alpha-L-Fuc-(1-6))-D-GlcNAc + H2O
-
655691
Thermus sp. Y5
alpha-L-fucose + beta-D-GlcNAc-(1-4)-D-GlcNAc
-
-
-
?
3.2.1.51
methyl alpha-L-fucopyranoside + H2O
or methanol or ethanol as acceptor
655691
Thermus sp.
methanol + alpha-L-fucose
-
-
-
?
3.2.1.51
methyl alpha-L-fucopyranoside + H2O
or methanol or ethanol as acceptor
655691
Thermus sp. Y5
methanol + alpha-L-fucose
-
-
-
?
3.2.1.51
additional information
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
655691
Thermus sp.
?
-
-
-
-
3.2.1.51
additional information
not: p-nitrophenyl beta-D-fucoside, p-nitrophenyl beta-L-fucopyranoside, p-nitrophenyl N-acetyl-beta-D-glucosaminide, p-nitrophenyl alpha-mannopyranoside
655691
Thermus sp. Y5
?
-
-
-
-
3.2.1.51
p-nitrophenyl alpha-L-fucopyranoside + H2O
or methanol or ethanol as acceptor
655691
Thermus sp.
p-nitrophenol + alpha-L-fucopyranose
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
3.2.1.51
tetramer
4 * 61000, SDS-PAGE
Thermus sp.
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
3.2.1.51
50
-
optimal temperatures for hydrolysis
Thermus sp.
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
3.2.1.51
37
-
the enzyme is stable overnight in the pH range from 5.5 to 7.5
Thermus sp.
3.2.1.51
45
-
thermal inactivation at temperatures above 45°C
Thermus sp.
3.2.1.51
60
-
the activity decreases rapidly at temperatures above 60°C
Thermus sp.
pH Stability (protein specific)
EC Number
pH Stability
pH Stability Maximum
Commentary
Organism
3.2.1.51
5.5
7.5
the enzyme is stable overnight at 37°C
Thermus sp.
pI Value (protein specific)
EC Number
Organism
Commentary
pI Value Maximum
pI Value
3.2.1.51
Thermus sp.
-
-
4.2