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Literature summary extracted from

  • Berteau, O.; McCort, I.; Goasdoue, N.; Tissot, B.; Daniel, R.
    Characterization of a new alpha-L-fucosidase isolated from the marine mollusk Pecten maximus that catalyzes the hydrolysis of alpha-L-fucose from algal fucoidan (Ascophyllum nodosum) (2002), Glycobiology, 12, 273-282.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.51 EDTA
-
 Pecten maximus
3.2.1.51 additional information the enzyme activity is increases with the ionic strength from 10 mM to 200 mM Pecten maximus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.51 1-deoxyfuconojirimycin
-
 Pecten maximus
3.2.1.51 1-deoxygalactonojirimycin
-
 Pecten maximus
3.2.1.51 1-deoxymannojirimycin
-
 Pecten maximus
3.2.1.51 1-deoxynojirimycin
-
 Pecten maximus
3.2.1.51 6-N-acetylamino-deoxymannojirimycin
-
 Pecten maximus
3.2.1.51 Cu2+
-
 Pecten maximus
3.2.1.51 Hg2+
-
 Pecten maximus
3.2.1.51 L-fucosamine
-
 Pecten maximus
3.2.1.51 L-fucose
-
 Pecten maximus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.51 0.65
-
 p-nitrophenyl alpha-L-fucopyranoside pH 4, 60°C Pecten maximus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.51 Zn2+ 150% activity at 1 mM Pecten maximus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.51 50000
-
 4 * 50000, SDS-PAGE Pecten maximus
3.2.1.51 200000
-
 gel filtration Pecten maximus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.2.1.51 additional information Pecten maximus the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.51 Pecten maximus
-
 marine mollusk
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.51
-
 Pecten maximus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.51 86
-
-
 Pecten maximus

Storage Stability

EC Number Storage Stability Organism
3.2.1.51 30°C, 30% loss of activity after 40 days Pecten maximus
3.2.1.51 60°C, 40% loss of activity after 1 day Pecten maximus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.51 additional information the enzyme is able to catalyse the hydrolysis of fucosyl units of Ascophyllum nodosum fucoidan Pecten maximus ?
-
 ?
3.2.1.51 p-nitrophenyl alpha-L-fucopyranoside + H2O
-
 Pecten maximus p-nitrophenol + alpha-L-fucopyranose anomeric configuration of the produced fucopyranse ?

Subunits

EC Number Subunits Comment Organism
3.2.1.51 tetramer 4 * 50000, SDS-PAGE Pecten maximus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.51 60
-
-
 Pecten maximus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.51 60
-
 40% of activity remains after 1 day Pecten maximus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.51 4
-
-
 Pecten maximus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.51 3 5.5 at pH 3.0 and pH 5.5: more than 50% of the fucosidase activity Pecten maximus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.2.1.51 0.000026
-
 1-deoxyfuconojirimycin pH 4, 60°C Pecten maximus
3.2.1.51 0.034
-
 1-deoxymannojirimycin pH 4, 60°C Pecten maximus
3.2.1.51 0.055
-
 6-N-acetylamino-deoxymannojirimycin pH 4, 60°C Pecten maximus
3.2.1.51 0.24
-
 L-fucose pH 4, 60°C Pecten maximus
3.2.1.51 0.316
-
 L-fucosamine pH 4, 60°C Pecten maximus
3.2.1.51 1
-
 1-deoxynojirimycin pH 4, 60°C Pecten maximus
3.2.1.51 1
-
 1-deoxygalactonojirimycin pH 4, 60°C Pecten maximus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.2.1.51 Pecten maximus isoelectric focusing
-
 6