Literature summary extracted from

Fabrega, S.; Durand, P.; Codogno, P.; Bauvy, C.; Delomenie, C.; Henrissat, B.; Martin, B.M.; McKinney, C.; Ginns, E.I.; Mornon, J.P.; Lehn, P.
Human glucocerebrosidase: heterologous expression of active site mutants in murine null cells (2000), Glycobiology, 10, 1217-1224.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
3.2.1.45	expression of mutant and wild-type enzymes in murine cells	Homo sapiens

Protein Variants

EC Number	Protein Variants	Comment	Organism
3.2.1.45	E235A	the mutant is catalytically inactive	Homo sapiens
3.2.1.45	E340A	the mutant is catalytically inactive	Homo sapiens

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.2.1.45	2	-	4-methylumbelliferyl-beta-D-glucopyranoside	pH 5.9	Homo sapiens

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.2.1.45	Homo sapiens	-	-	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.2.1.45	0.00007	-	mutant E235A	Homo sapiens
3.2.1.45	0.00008	-	mutant E340A	Homo sapiens
3.2.1.45	0.0046	-	wild-type enzyme	Homo sapiens

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.2.1.45	4-methylumbelliferyl-beta-D-glucopyranoside + H2O	-	Homo sapiens	4-methylumbelliferone + beta-D-glucose	-	?

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Release 2023.1 (January 2023)