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Literature summary extracted from

  • Castro, H.; Budde, H.; Flohe, L.; Hofmann, B.; Lunsdorf, H.; Wissing, J.; Tomas, A.M.
    Specificity and kinetics of a mitochondrial peroxiredoxin of Leishmania infantum (2002), Free Radic. Biol. Med., 33, 1563-1574.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.11.1.24 expression in Escherichia coli as an N-terminally His-tagged protein Leishmania infantum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.24 0.0091
-
 tert-butyl hydroperoxide pH 7.6, 25°C Leishmania infantum
1.11.1.24 0.0319
-
 tryparedoxin 2 pH 7.6, 25°C Leishmania infantum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.11.1.24 mitochondrion
-
 Leishmania infantum 5739
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.24 27345
-
 10 * 27345, the enzyme exists as a homodecameric ring structure composed of five dimers, MALDI-TOF spectrometry Leishmania infantum

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.24 Leishmania infantum
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.24 cumene hydroperoxide + tryparedoxin 2 57% of the activity with H2O2 Leishmania infantum 2-phenylpropan-2-ol + oxidized tryparedoxin 2
-
 ?
1.11.1.24 H2O2 + tryparedoxin 2
-
 Leishmania infantum H2O + oxidized tryparedoxin 2
-
 ?
1.11.1.24 linoleic acid hydroperoxide + tryparedoxin 2 7.8% of the activity with H2O2 Leishmania infantum ?
-
 ?
1.11.1.24 additional information tryparedoxin peroxidase activity. The enzyme does not follow a classic ping-pong mechanism Leishmania infantum ?
-
 ?
1.11.1.24 phosphatidyl choline hydroperoxide + tryparedoxin 2 3.8% of the activity with H2O2 Leishmania infantum ?
-
 ?
1.11.1.24 tert-butyl hydroperoxide + reduced thioredoxin 9.5% of the activity with tert-butyl hydroperoxide and tryparedoxin 2 Leishmania infantum tert-butanol + oxidized thioredoxin + H2O
-
 ?
1.11.1.24 tert-butyl hydroperoxide + tryparedoxin 2 95% of the activity with H2O2 Leishmania infantum tert-butanol + oxidized tryparedoxin 2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.24 decamer 10 * 27345, the enzyme exists as a homodecameric ring structure composed of five dimers, MALDI-TOF spectrometry Leishmania infantum

Synonyms

EC Number Synonyms Comment Organism
1.11.1.24 LimTXNPx
-
 Leishmania infantum
1.11.1.24 peroxiredoxin
-
 Leishmania infantum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.11.1.24 37
-
 tryparedoxin 2 pH 7.6, 25°C Leishmania infantum