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Literature summary extracted from

  • Choi, Y.K.; Jun, S.R.; Cha, E.Y.; Park, J.S.; Park, Y.S.
    Sepiapterin reductases from Chlorobium tepidum and Chlorobium limicola catalyze the synthesis of L-threo-tetrahydrobiopterin from 6-pyruvoyltetrahydropterin (2005), FEMS Microbiol. Lett., 242, 95-99.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.153 expressed in Escherichia coli BL21(DE3)/pLysS Chlorobaculum tepidum
1.1.1.153 expressed in Escherichia coli BL21(DE3)/pLysS Chlorobium limicola

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ Chlorobaculum tepidum final step in the synthesis of tetrahydrobiopterin L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ Chlorobium limicola final step in the synthesis of tetrahydrobiopterin L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.153 Chlorobaculum tepidum
-
-
-
1.1.1.153 Chlorobium limicola Q5XPX7
-
-
1.1.1.153 Chlorobium limicola Q5XPX7 complete ORF
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.153 recombinant protein using His-tag Chlorobaculum tepidum
1.1.1.153 recombinant protein using His-tag Chlorobium limicola

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
-
 Chlorobaculum tepidum L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+
-
 Chlorobium limicola L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ final step in the synthesis of tetrahydrobiopterin Chlorobaculum tepidum L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
1.1.1.153 6-pyruvoyltetrahydropterin + 2 NADPH + 2 H+ final step in the synthesis of tetrahydrobiopterin Chlorobium limicola L-threo-tetrahydrobiopterin + 2 NADP+ no formation of the L-erythro isomer ?
1.1.1.153 sepiapterin + NADPH + H+
-
 Chlorobaculum tepidum 7,8-dihydrobiopterin + NADP+
-
 ?
1.1.1.153 sepiapterin + NADPH + H+
-
 Chlorobium limicola 7,8-dihydrobiopterin + NADP+
-
 ?

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.153 NADPH
-
 Chlorobaculum tepidum
1.1.1.153 NADPH
-
 Chlorobium limicola