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Literature summary extracted from

  • Yuan, B.; Yoshikane, Y.; Yokochi, N.; Ohnishi, K.; Yagi, T.
    The nitrogen-fixing symbiotic bacterium Mesorhizobium loti has and expresses the gene encoding pyridoxine 4-oxidase involved in the degradation of vitamin B6 (2004), FEMS Microbiol. Lett., 234, 225-230.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.3.12
-
 Mesorhizobium loti

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.3.12 0.078
-
 O2 pH 8.0, 30°C Mesorhizobium loti
1.1.3.12 0.213
-
 pyridoxine pH 8.0, 30°C Mesorhizobium loti

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.3.12 53000
-
 1 * 53000, SDS-PAGE, 1 * 55000, deduced from gene sequence Mesorhizobium loti
1.1.3.12 54500
-
 gel filtration Mesorhizobium loti
1.1.3.12 55000
-
 1 * 53000, SDS-PAGE, 1 * 55000, deduced from gene sequence Mesorhizobium loti

Organism

EC Number Organism UniProt Comment Textmining
1.1.3.12 Mesorhizobium loti
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.3.12
-
 Mesorhizobium loti

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.3.12 19.4
-
 pH 8.0, 30°C Mesorhizobium loti

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.3.12 2,6-dihydroxypyridine + O2 0.04% of the rate with pyridoxine Mesorhizobium loti ? + H2O2
-
 ?
1.1.3.12 4-pyridinemethanol + O2 0.12% of the rate with pyridoxine Mesorhizobium loti isonicotinaldehyde + H2O2
-
 ?
1.1.3.12 additional information no substrates are: pyridoxamine, pyridoxal 5’-phosphate, pyridoxamine 5’-phosphate, benzyl alcohol, 2-hydroxybenzyl alcohol Mesorhizobium loti ?
-
 ?
1.1.3.12 pyridoxine + O2
-
 Mesorhizobium loti pyridoxal + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.3.12 monomer 1 * 53000, SDS-PAGE, 1 * 55000, deduced from gene sequence Mesorhizobium loti

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.3.12 40
-
-
 Mesorhizobium loti

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.3.12 8 8.5
-
 Mesorhizobium loti

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.3.12 FAD
-
 Mesorhizobium loti