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Literature summary extracted from

  • Ro, Y.T.; Lee, H.I.; Kim, E.J.; Koo, J.H.; Kim, E.; Kim, Y.M.
    Purification, characterization, and physiological response of a catalase-peroxidase in Mycobacterium sp. strain JC1 DSM 3803 grown on methanol (2003), FEMS Microbiol. Lett., 226, 397-403.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.11.1.6 3-Amino-1,2,4-triazole 18% activation of catalase activity and unaltered peroxidase activity at 0.1 mM, 56% activation of catalase activity and 21% activation of peroxidase activity at 10 mM Mycobacterium sp.
1.11.1.21 3-Amino-1,2,4-triazole 156% relative catalase activity at 10 mM, 121% relative peroxidase activity at 10 mM Mycobacterium sp.

General Stability

EC Number General Stability Organism
1.11.1.6 enzyme is still active in 0.1% SDS Mycobacterium sp.
1.11.1.21 the enzyme retains both catalase and peroxidase activities after having been subjected to PAGE in the presence of 0.1% (w/v) SDS, over 90% of the initial catalase and peroxidase activities are detected from enzyme preparations incubated for 5 min in an ethanol-chloroform mixture Mycobacterium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.11.1.6 hydroxylamine 99% inhibition of catalase activity and 17% inhibition of peroxidase activity at 0.1 mM, complete inhibition of catalase activity and 56% inhibition of peroxidase activity at 1 mM Mycobacterium sp.
1.11.1.6 KCN 74% inhibition of catalase activity and 86% inhibition of peroxidase activity at 0.1 mM, 96% inhibition of catalase and peroxidase activity at 1 mM Mycobacterium sp.
1.11.1.6 NaN3 68% inhibition of catalase activity and 90% inhibition of peroxidase activity at 0.1 mM, 92% inhibition of catalase activity and 99% inhibition of peroxidase activity at 1 mM Mycobacterium sp.
1.11.1.21 hydroxylamine complete inhibition of catalase activity at 1 mM, 42% residual peroxidase activity at 1 mM Mycobacterium sp.
1.11.1.21 isoniazid
-
 Mycobacterium sp.
1.11.1.21 KCN 4% residual catalase activity at 1 mM, 4% residual peroxidase activity at 1 mM Mycobacterium sp.
1.11.1.21 additional information the enzyme is resistant to inhibition by 3-amino-1,2,4-triazole Mycobacterium sp.
1.11.1.21 NaN3 8% residual catalase activity at 1 mM, 1% residual peroxidase activity at 1 mM Mycobacterium sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.11.1.6 0.048
-
 o-Dianisidine peroxidase activity, pH 4.5, 30°C Mycobacterium sp.
1.11.1.6 1.47
-
 H2O2 peroxidase activity, pH 4.5, 30°C Mycobacterium sp.
1.11.1.6 6.3
-
 H2O2 catalase activity, pH 7.0, 30°C Mycobacterium sp.
1.11.1.21 0.0478
-
 o-Dianisidine peroxidase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C Mycobacterium sp.
1.11.1.21 1.47
-
 H2O2 peroxidase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C Mycobacterium sp.
1.11.1.21 6.3
-
 H2O2 catalase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C Mycobacterium sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.11.1.6 Mn2+ required for catalase activity Mycobacterium sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.11.1.6 80000
-
 2 * 80000, SDS-PAGE Mycobacterium sp.
1.11.1.6 160000
-
 gel filtration Mycobacterium sp.
1.11.1.21 80000
-
 2 * 80000, SDS-PAGE Mycobacterium sp.
1.11.1.21 160000
-
 gel filtration Mycobacterium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.11.1.6 Mycobacterium sp.
-
-
-
1.11.1.6 Mycobacterium sp. DSM 3803 / JC1
-
-
-
1.11.1.21 Mycobacterium sp.
-
 formerly Acinetobacter sp.
-
1.11.1.21 Mycobacterium sp. JC1 DSM 3803
-
 formerly Acinetobacter sp.
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.11.1.6 native enzyme from the soluble fraction, 47.3fold to homogeneity Mycobacterium sp.
1.11.1.21 ammonium sulfate precipitation, Sepharose 6B column chromatography, DEAE-Sephacel column chromatography, Sephadex G-25 gel filtration, and hydroxylapatite column chromatography Mycobacterium sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.11.1.6 culture condition:methanol-grown cell 0.5% v/v methanol in the cell culture medium Mycobacterium sp.
-
1.11.1.21 culture condition:methanol-grown cell
-
 Mycobacterium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.11.1.6 additional information
-
-
 Mycobacterium sp.
1.11.1.6 1145
-
 purified enzyme, catalase activity Mycobacterium sp.
1.11.1.21 24.2
-
 crude extract, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C Mycobacterium sp.
1.11.1.21 1145
-
 after 47.3fold purification, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C Mycobacterium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.11.1.6 H2O2 catalase activity Mycobacterium sp. O2 + H2O
-
 ?
1.11.1.6 H2O2 catalase activity Mycobacterium sp. DSM 3803 / JC1 O2 + H2O
-
 ?
1.11.1.6 H2O2 catalase activity Mycobacterium sp. JC1 DSM 3803 O2 + H2O
-
 ?
1.11.1.6 H2O2 + methanol peroxidase activity Mycobacterium sp. formaldehyde + H2O
-
 ?
1.11.1.6 H2O2 + methanol peroxidase activity Mycobacterium sp. DSM 3803 / JC1 formaldehyde + H2O
-
 ?
1.11.1.6 H2O2 + methanol peroxidase activity Mycobacterium sp. JC1 DSM 3803 formaldehyde + H2O
-
 ?
1.11.1.6 H2O2 + o-dianisidine peroxidase activity Mycobacterium sp. ? + H2O
-
 ?
1.11.1.6 H2O2 + o-dianisidine peroxidase activity Mycobacterium sp. DSM 3803 / JC1 ? + H2O
-
 ?
1.11.1.6 H2O2 + o-dianisidine peroxidase activity Mycobacterium sp. JC1 DSM 3803 ? + H2O
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium sp. O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium sp. DSM 3803 / JC1 O2 + H2O
-
 ?
1.11.1.21 H2O2
-
 Mycobacterium sp. JC1 DSM 3803 O2 + H2O
-
 ?
1.11.1.21 methanol + H2O2 at pH 4.5 Mycobacterium sp. formaldehyde + H2O
-
 ?
1.11.1.21 NADH + H2O2
-
 Mycobacterium sp. ?
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Mycobacterium sp. oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Mycobacterium sp. DSM 3803 / JC1 oxidized o-dianisidine + H2O
-
 ?
1.11.1.21 o-dianisidine + H2O2
-
 Mycobacterium sp. JC1 DSM 3803 oxidized o-dianisidine + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.11.1.6 dimer 2 * 80000, SDS-PAGE Mycobacterium sp.
1.11.1.21 homodimer 2 * 80000, SDS-PAGE Mycobacterium sp.

Synonyms

EC Number Synonyms Comment Organism
1.11.1.6 catalase-peroxidase
-
 Mycobacterium sp.
1.11.1.6 CP
-
 Mycobacterium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.11.1.6 30
-
 catalase activity Mycobacterium sp.
1.11.1.6 40
-
 peroxidase activity Mycobacterium sp.
1.11.1.21 30
-
 peroxidase activity Mycobacterium sp.
1.11.1.21 40
-
 catalase activity Mycobacterium sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.11.1.6 60
-
 1 min, loss of over 80% of both activities Mycobacterium sp.
1.11.1.21 60
-
 when the purified enzyme is incubated for 1 min at 60°C, catalase and peroxidase activities decrease by over 80% Mycobacterium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.11.1.6 4
-
 peroxidase activity Mycobacterium sp.
1.11.1.6 7
-
 catalase activity Mycobacterium sp.
1.11.1.21 4
-
 peroxidase activity Mycobacterium sp.
1.11.1.21 7
-
 catalase activity Mycobacterium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.11.1.21 heme
-
 Mycobacterium sp.

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.11.1.6 Mycobacterium sp. isoelectric focusing
-
 4.9
1.11.1.21 Mycobacterium sp. isoelectric focusing
-
 4.8

Expression

EC Number Organism Comment Expression
1.11.1.21 Mycobacterium sp. the peroxidase activity of catalase-peroxidase in cells growing at the late-exponential growth phase (24 h after inoculation) decreases to 25% less than that in cells growing in the absence of isoniazid down
1.11.1.21 Mycobacterium sp. isoniazid had almost no effect on the expression of catalase-peroxidase additional information
1.11.1.21 Mycobacterium sp. the peroxidase activity of catalase-peroxidase in sodium nitroprusside-treated cells is 2.7fold higher than that in cells not treated with 1 mM sodium nitroprusside up