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Literature summary extracted from

  • Polcyn, W.; Lucinski, R.
    Aerobic and anaerobic nitrate and nitrite reduction in free-living cells of Bradyrhizobium sp. (Lupinus) (2003), FEMS Microbiol. Lett., 226, 331-337.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.7.1.3 additional information growth stimulation is higher on nitrate than on nitrite Bradyrhizobium sp.
1.7.1.3 NH4+ slight activation of the enzyme under anaerobic conditions Bradyrhizobium sp.
1.7.1.4 nitrate 2fold enhanced induction independently of the NH4+ concentration, growth stimulation is higher on nitrate than on nitrite Bradyrhizobium sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.7.1.3 additional information intracellularly accumulated nitrite can inhibit nitrate uptake, addition of nitrite at concentrations above 5 mM is toxic and causes growth retardation Bradyrhizobium sp.
1.7.1.3 NH4+ inhibits the enzyme under aerobic conditions Bradyrhizobium sp.
1.7.1.4 additional information intracellularly accumulated nitrite can inhibit nitrate uptake, addition of nitrite at concentrations above 5 mM is toxic and causes growth retardation Bradyrhizobium sp.
1.7.1.4 NH4+ inhibits under aerobic and anaerobic conditions Bradyrhizobium sp.
1.7.1.4 O2 enzyme activity under anaerobic conditions is 2fold higher than under aerobic conditions Bradyrhizobium sp.

Organism

EC Number Organism UniProt Comment Textmining
1.7.1.3 Bradyrhizobium sp.
-
 Lupinus, strain USDA 3045
-
1.7.1.4 Bradyrhizobium sp.
-
 Lupinus, strain USDA 3045
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.7.1.3 cell culture free-living cells, yeast extract/mannitol medium Bradyrhizobium sp.
-
1.7.1.4 cell culture free-living cells, yeast extract/mannitol medium Bradyrhizobium sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.7.1.3 0.02
-
 activity under aerobic conditions in presence of NH4+ Bradyrhizobium sp.
1.7.1.3 0.1
-
 activity under aerobic conditions Bradyrhizobium sp.
1.7.1.3 0.3
-
 activity under anaerobic conditions Bradyrhizobium sp.
1.7.1.3 0.37
-
 activity under anaerobic conditions in presence of NH4+ Bradyrhizobium sp.
1.7.1.4 0.06
-
 activity under aerobic conditions in presence of NH4+ Bradyrhizobium sp.
1.7.1.4 0.1
-
 activity under aerobic conditions Bradyrhizobium sp.
1.7.1.4 0.13
-
 activity under anaerobic conditions in presence of NH4+ Bradyrhizobium sp.
1.7.1.4 0.18
-
 activity under anaerobic conditions Bradyrhizobium sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.7.1.3 nitrate + reduced benzyl viologen
-
 Bradyrhizobium sp. nitrite + benzyl viologen
-
 ?
1.7.1.4 nitrite + reduced benzyl viologen
-
 Bradyrhizobium sp. ammonium hydroxide + H2O + benzyl viologen
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.7.1.3 nitrate reductase
-
 Bradyrhizobium sp.
1.7.1.3 NR
-
 Bradyrhizobium sp.
1.7.1.4 NiR
-
 Bradyrhizobium sp.
1.7.1.4 nitrite reductase
-
 Bradyrhizobium sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.7.1.3 additional information
-
 a growth temperature above 20°C is required Bradyrhizobium sp.
1.7.1.3 30
-
 assay at Bradyrhizobium sp.
1.7.1.4 30
-
 assay at Bradyrhizobium sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.7.1.3 7.5
-
 assay at Bradyrhizobium sp.
1.7.1.4 7.5
-
 assay at Bradyrhizobium sp.

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.1.3 benzyl viologen
-
 Bradyrhizobium sp.
1.7.1.4 benzyl viologen
-
 Bradyrhizobium sp.