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Literature summary extracted from

  • Hansen, T.; Schlichting, B.; Schonheit, P.
    Glucose-6-phosphate dehydrogenase from the hyperthermophilic bacterium Thermotoga maritima: expression of the g6pd gene and characterization of an extremely thermophilic enzyme (2002), FEMS Microbiol. Lett., 216, 249-253.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.49 DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli strain BL21 as His-tagged enzyme Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.49 ATP 15% inhibition at 2.5 mM, 24% inhibition at 10 mM Thermotoga maritima
1.1.1.49 NADPH competitive Thermotoga maritima
1.1.1.49 phosphoenolpyruvate 13% inhibition at 2.5 mM, 31% inhibition at 10 mM Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.49 0.03
-
 NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 0.06
-
 thio-NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 0.15
-
 D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 1.5
-
 thio-NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 12
-
 NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 35
-
 2-deoxy-D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.49 95000
-
 gel filtration, recombinant enzyme Thermotoga maritima

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.49 D-glucose 6-phosphate + NADP+ Thermotoga maritima
-
 D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.49 Thermotoga maritima
-
 gene g6pd
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.49 recombinant His-tagged enzyme from Escherichia coli by nickel affinity chromatography Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.49 2-deoxy-D-glucose 6-phosphate + NAD(P)+ low activity Thermotoga maritima 2-deoxy-D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
 ?
1.1.1.49 D-glucose 6-phosphate + NAD(P)+
-
 Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NAD(P)H
-
 ?
1.1.1.49 D-glucose 6-phosphate + NAD+
-
 Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NADH + H+
-
 ?
1.1.1.49 D-glucose 6-phosphate + NADP+
-
 Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + NADPH + H+
-
 ?
1.1.1.49 D-glucose 6-phosphate + thio-NAD(P)+ the thio-cofactors give increased activity compared to the non-thio-cofactors Thermotoga maritima D-glucono-1,5-lactone 6-phosphate + thio-NAD(P)H
-
 ?
1.1.1.49 D-glucose 6-phosphate + thio-NAD+
-
 Thermotoga maritima 6-phospho-D-glucono-1,5-lactone + thio-NADH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.49 dimer 2 x 60000, alpha2, recombinant enzyme, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
1.1.1.49 G6PD
-
 Thermotoga maritima
1.1.1.49 glucose-6-phosphate dehydrogenase
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.49 92
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.49 40 95
-
 Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.49 70
-
 120 min, no loss in activity Thermotoga maritima
1.1.1.49 80
-
 120 min, 20% loss in activity Thermotoga maritima
1.1.1.49 95 100 120 min, almost complete loss in activity Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.49 1100
-
 NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 1800
-
 2-deoxy-D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 2100
-
 thio-NAD+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 3500
-
 NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 3500
-
 D-glucose 6-phosphate pH 7.4, 80°C, recombinant enzyme Thermotoga maritima
1.1.1.49 8000
-
 thio-NADP+ pH 7.4, 80°C, recombinant enzyme Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.49 7.4
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.49 5.9 9 50% of maximal activity at pH 5.9 and pH 9.0 Thermotoga maritima

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.49 NAD+
-
 Thermotoga maritima
1.1.1.49 NADP+ highly preferred cofactor, 1000fold higher activity than with NAD+ Thermotoga maritima
1.1.1.49 thio-NAD+
-
 Thermotoga maritima
1.1.1.49 thio-NADP+ best cofactor Thermotoga maritima

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
1.1.1.49 0.11
-
 NADPH pH 7.4, 80°C, recombinant enzyme Thermotoga maritima