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Literature summary extracted from

  • Inoue, H.; Tamura, T.; Ehara, N.; Nishito, A.; Nakayama, Y.; Maekawa, M.; Imada, K.; Tanaka, H.; Inagaki, K.
    Biochemical and molecular characterization of the NAD(+)-dependent isocitrate dehydrogenase from the chemolithotroph Acidithiobacillus thiooxidans (2002), FEMS Microbiol. Lett., 214, 127-132.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.41 gene icd, DNA and amino acid sequence determination and analysis, expression in enzyme-deficient Escherichia coli strain EB106 Acidithiobacillus thiooxidans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.41 additional information
-
additional information kinetic properties of the native and the recombinant enzyme are similar Acidithiobacillus thiooxidans
1.1.1.41 0.18
-
NAD+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans
1.1.1.41 6.57
-
NADP+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.41 Mg2+
-
Acidithiobacillus thiooxidans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.1.1.41 45000
-
2 * 45000, SDS-PAGE Acidithiobacillus thiooxidans
1.1.1.41 90000
-
gel filtration and native PAGE Acidithiobacillus thiooxidans

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.41 isocitrate + NAD+ Acidithiobacillus thiooxidans
-
2-oxoglutarate + CO2 + NADH + H+
-
?
1.1.1.41 isocitrate + NAD+ Acidithiobacillus thiooxidans ON107
-
2-oxoglutarate + CO2 + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.41 Acidithiobacillus thiooxidans Q8GAX0
-
-
1.1.1.41 Acidithiobacillus thiooxidans ON107 Q8GAX0
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.41 multi-step purification of the native enzyme, 520fold to homogeneity, partial purification of the recombinant enzyme from Escherichia coli Acidithiobacillus thiooxidans

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.41 120
-
purified native enzyme Acidithiobacillus thiooxidans

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.41 isocitrate + NAD+
-
Acidithiobacillus thiooxidans 2-oxoglutarate + CO2 + NADH + H+
-
?
1.1.1.41 isocitrate + NAD+
-
Acidithiobacillus thiooxidans ON107 2-oxoglutarate + CO2 + NADH + H+
-
?
1.1.1.41 isocitrate + NADP+
-
Acidithiobacillus thiooxidans 2-oxoglutarate + CO2 + NADPH + H+
-
r
1.1.1.41 isocitrate + NADP+
-
Acidithiobacillus thiooxidans ON107 2-oxoglutarate + CO2 + NADPH + H+
-
r

Subunits

EC Number Subunits Comment Organism
1.1.1.41 dimer 2 * 45000, SDS-PAGE Acidithiobacillus thiooxidans

Synonyms

EC Number Synonyms Comment Organism
1.1.1.41 ICDH
-
Acidithiobacillus thiooxidans
1.1.1.41 NAD+-dependent isocitrate dehydrogenase
-
Acidithiobacillus thiooxidans

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.41 30
-
assay at Acidithiobacillus thiooxidans

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.41 55
-
stable up to Acidithiobacillus thiooxidans
1.1.1.41 70
-
inactivation after 30 min Acidithiobacillus thiooxidans

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.41 21.3
-
NADP+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans
1.1.1.41 45.1
-
NAD+ pH 9.0, 30°C, native enzyme Acidithiobacillus thiooxidans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.41 8.5
-
-
Acidithiobacillus thiooxidans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.41 6 10.2
-
Acidithiobacillus thiooxidans

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.41 NAD+ highly preferred cofactor, 78fold higher activity than with NADP+ Acidithiobacillus thiooxidans
1.1.1.41 NADP+ 78fold lower activity than with NAD+ Acidithiobacillus thiooxidans