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Literature summary extracted from

  • Martens, S.; Teeri, T.; Forkmann, G.
    Heterologous expression of dihydroflavonol 4-reductases from various plants (2002), FEBS Lett., 531, 453-458.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.219 functional expression in tobacco protoplasts via electroporation, subcloning and overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1, the recombinant Escherichia coli strain shows no enzyme activity Gerbera hybrid cultivar
1.1.1.219 overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Matthiola incana
1.1.1.219 overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Dianthus caryophyllus
1.1.1.219 overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Rosa hybrid cultivar
1.1.1.219 overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Solanum lycopersicum
1.1.1.219 overexpression in Escherichia coli strain GI724 and in Saccharomyces cerevisiae strain INV Sc1 Callistephus chinensis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.219 additional information Matthiola incana enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?
1.1.1.219 additional information Gerbera hybrid cultivar enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?
1.1.1.219 additional information Dianthus caryophyllus enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?
1.1.1.219 additional information Rosa hybrid cultivar enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?
1.1.1.219 additional information Solanum lycopersicum enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?
1.1.1.219 additional information Callistephus chinensis enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?
1.1.1.219 additional information Rosa hybrid cultivar Kardinal enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.219 Callistephus chinensis P51103 line 101
-
1.1.1.219 Dianthus caryophyllus P51104 Tanga
-
1.1.1.219 Gerbera hybrid cultivar P51105 v. Terra Regina
-
1.1.1.219 Matthiola incana
-
 line 10
-
1.1.1.219 no activity in Escherichia coli
-
-
-
1.1.1.219 no activity in Nicotiana tabacum
-
-
-
1.1.1.219 no activity in Saccharomyces cerevisiae
-
-
-
1.1.1.219 Rosa hybrid cultivar Q41158 Kardinal
-
1.1.1.219 Rosa hybrid cultivar Kardinal Q41158 Kardinal
-
1.1.1.219 Solanum lycopersicum P51107 line 19
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.219 flower
-
 Matthiola incana
-
1.1.1.219 flower
-
 Gerbera hybrid cultivar
-
1.1.1.219 flower
-
 Dianthus caryophyllus
-
1.1.1.219 flower
-
 Rosa hybrid cultivar
-
1.1.1.219 flower
-
 Solanum lycopersicum
-
1.1.1.219 flower
-
 Callistephus chinensis
-
1.1.1.219 leaf young Matthiola incana
-
1.1.1.219 leaf young Gerbera hybrid cultivar
-
1.1.1.219 leaf young Dianthus caryophyllus
-
1.1.1.219 leaf young Rosa hybrid cultivar
-
1.1.1.219 leaf young Solanum lycopersicum
-
1.1.1.219 leaf young Callistephus chinensis
-
1.1.1.219 petal
-
 Matthiola incana
-
1.1.1.219 petal
-
 Gerbera hybrid cultivar
-
1.1.1.219 petal
-
 Dianthus caryophyllus
-
1.1.1.219 petal
-
 Rosa hybrid cultivar
-
1.1.1.219 petal
-
 Solanum lycopersicum
-
1.1.1.219 petal
-
 Callistephus chinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.219 (2R,3R)-(+)-dihydrokaempferol + NADPH
-
 Matthiola incana (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-(+)-dihydrokaempferol + NADPH
-
 Gerbera hybrid cultivar (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-(+)-dihydrokaempferol + NADPH
-
 Dianthus caryophyllus (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-(+)-dihydrokaempferol + NADPH
-
 Callistephus chinensis (2R,3S,4S)-cis-3,4-leucopelargonidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Matthiola incana (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Gerbera hybrid cultivar (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Dianthus caryophyllus (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Rosa hybrid cultivar (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Solanum lycopersicum (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Callistephus chinensis (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydromyricetin + NADPH
-
 Rosa hybrid cultivar Kardinal (2R,3S,4R)-3,4-leucodelphinidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Matthiola incana (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Gerbera hybrid cultivar (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Dianthus caryophyllus (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Rosa hybrid cultivar (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Solanum lycopersicum (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Callistephus chinensis (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 (2R,3R)-dihydroquercetin + NADPH
-
 Rosa hybrid cultivar Kardinal (2R,3S,4R)-leucocyanidin + NADP+
-
 ?
1.1.1.219 additional information stereospecific reaction Callistephus chinensis ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Matthiola incana ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Gerbera hybrid cultivar ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Dianthus caryophyllus ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Rosa hybrid cultivar ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Solanum lycopersicum ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Callistephus chinensis ?
-
 ?
1.1.1.219 additional information no activity with (+)-dihydrokaempferol Rosa hybrid cultivar ?
-
 ?
1.1.1.219 additional information no activity with (+)-dihydrokaempferol Solanum lycopersicum ?
-
 ?
1.1.1.219 additional information enzyme is involved in production of leucoanthocyanidins, i.e flavan-3,4-diols, and in reduction of flavanones to flavan-4-ols which are important intermediates in the 3-deoxyflavonoid pathway Rosa hybrid cultivar Kardinal ?
-
 ?
1.1.1.219 additional information no activity with (+)-dihydrokaempferol Rosa hybrid cultivar Kardinal ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.219 DFR
-
 Matthiola incana
1.1.1.219 DFR
-
 Gerbera hybrid cultivar
1.1.1.219 DFR
-
 Dianthus caryophyllus
1.1.1.219 DFR
-
 Rosa hybrid cultivar
1.1.1.219 DFR
-
 Solanum lycopersicum
1.1.1.219 DFR
-
 Callistephus chinensis
1.1.1.219 dihydroflavonol 4-reductase
-
 Matthiola incana
1.1.1.219 dihydroflavonol 4-reductase
-
 Gerbera hybrid cultivar
1.1.1.219 dihydroflavonol 4-reductase
-
 Dianthus caryophyllus
1.1.1.219 dihydroflavonol 4-reductase
-
 Rosa hybrid cultivar
1.1.1.219 dihydroflavonol 4-reductase
-
 Solanum lycopersicum
1.1.1.219 dihydroflavonol 4-reductase
-
 Callistephus chinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.219 30
-
 assay at Matthiola incana
1.1.1.219 30
-
 assay at Gerbera hybrid cultivar
1.1.1.219 30
-
 assay at Dianthus caryophyllus
1.1.1.219 30
-
 assay at Rosa hybrid cultivar
1.1.1.219 30
-
 assay at Solanum lycopersicum
1.1.1.219 30
-
 assay at Callistephus chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.219 7.5
-
 assay at Matthiola incana
1.1.1.219 7.5
-
 assay at Gerbera hybrid cultivar
1.1.1.219 7.5
-
 assay at Dianthus caryophyllus
1.1.1.219 7.5
-
 assay at Rosa hybrid cultivar
1.1.1.219 7.5
-
 assay at Solanum lycopersicum
1.1.1.219 7.5
-
 assay at Callistephus chinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.219 NADPH
-
 Matthiola incana
1.1.1.219 NADPH
-
 Gerbera hybrid cultivar
1.1.1.219 NADPH
-
 Dianthus caryophyllus
1.1.1.219 NADPH
-
 Rosa hybrid cultivar
1.1.1.219 NADPH
-
 Solanum lycopersicum
1.1.1.219 NADPH
-
 Callistephus chinensis