Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Ullrich, A.; Knecht, W.; Piskur, J.; Loffler, M.
    Plant dihydroorotate dehydrogenase differs significantly in substrate specificity and inhibition from the animal enzymes (2002), FEBS Lett., 529, 346-350.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.2 DELTA1-75 N-terminally truncated enzyme, drastic increase in kcat- and KM-value Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.2 0.04
-
 dihydroorotate pH 8.0, 30°C, wild-type Arabidopsis thaliana
1.3.5.2 0.112
-
 decylubiquinone pH 8.0, 30°C, wild-type Arabidopsis thaliana
1.3.5.2 0.121
-
 dihydroorotate pH 8.0, 30°C, DELTA1-75 mutant Arabidopsis thaliana
1.3.5.2 0.341
-
 decylubiquinone pH 8.0, 30°C, DELTA1-75 mutant Arabidopsis thaliana

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.2 48500
-
 x * 48500, deduced from gene sequendce Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.2 Arabidopsis thaliana P32746
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.3.5.2 (S)-dihydroorotate + a quinone = orotate + a quinol two-site ping-pong mechanism Arabidopsis thaliana
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.2 dihydroorotate + 2,5-dimethyl-p-benzoquinone 95% the rate of decylubiquinone, wild-type, 75% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced 2,5-dimethyl-p-benzoquinone
-
 ?
1.3.5.2 dihydroorotate + CoQ0 102% the rate of decylubiquinone, wild-type, 67% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced CoQo
-
 ?
1.3.5.2 dihydroorotate + decylplastoquinone 95% the rate of decylubiquinone, wild-type, 104% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced decylplastoquinone
-
 ?
1.3.5.2 dihydroorotate + decylubiquinone
-
 Arabidopsis thaliana orotate + reduced decylubiquinone
-
 ?
1.3.5.2 dihydroorotate + menadione 82% the rate of decylubiquinone, wild-type, 78% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced menadione
-
 ?
1.3.5.2 dihydroorotate + ubiquinone-50 83% the rate of decylubiquinone, wild-type, 76% the rate of decylubiquinone, DELTA1-75 mutant Arabidopsis thaliana orotate + reduced ubiquinone-50
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.2 ? x * 48500, deduced from gene sequendce Arabidopsis thaliana

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.2 8.2
-
 dihydroorotate pH 8.0, 30°C, wild-type Arabidopsis thaliana
1.3.5.2 72.9
-
 dihydroorotate pH 8.0, 30°C, DELTA1-75 mutant Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.3.5.2 7.5
-
-
 Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.2 flavin 0.8-1.1 mol per mol of protein Arabidopsis thaliana