EC Number | Cloned (Comment) | Organism |
---|---|---|
3.6.1.23 | expressed in Escherichia coli strain BW286 | Trypanosoma cruzi |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine | - |
Trypanosoma cruzi | |
3.6.1.23 | alpha,beta-imido-dUDP | - |
Trypanosoma cruzi |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.0005 | - |
dUTP | 25°C | Trypanosoma cruzi | |
3.6.1.23 | 0.0062 | - |
dUDP | 25°C | Trypanosoma cruzi |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.6.1.23 | Mg2+ | required | Trypanosoma cruzi |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.6.1.23 | 32000 | - |
2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence | Trypanosoma cruzi |
3.6.1.23 | 32065 | - |
2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence | Trypanosoma cruzi |
3.6.1.23 | 62440 | - |
gel filtration | Trypanosoma cruzi |
3.6.1.23 | 64000 | - |
SDS-PAGE of cross-linked subunits | Trypanosoma cruzi |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUTP + H2O | Trypanosoma cruzi | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | dUMP + diphosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.6.1.23 | Trypanosoma cruzi | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.6.1.23 | recombinant protein | Trypanosoma cruzi |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.6.1.23 | dUDP + H2O | - |
Trypanosoma cruzi | dUMP + phosphate | - |
? | |
3.6.1.23 | dUTP + H2O | - |
Trypanosoma cruzi | dUMP + diphosphate | - |
? | |
3.6.1.23 | dUTP + H2O | removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA | Trypanosoma cruzi | dUMP + diphosphate | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.6.1.23 | dimer | 2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence | Trypanosoma cruzi |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 2.8 | - |
dUTP | 25°C | Trypanosoma cruzi |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.6.1.23 | 0.00024 | - |
alpha,beta-imido-dUDP | 25°C | Trypanosoma cruzi | |
3.6.1.23 | 2.5 | - |
5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine | 25°C | Trypanosoma cruzi |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.6.1.23 | Trypanosoma cruzi | calculated from amino acid sequence | - |
4.9 |