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Literature summary extracted from

  • Bernier-Villamor, V.; Camacho, A.; Hidalgo-Zarco, F.; Perez, J.; Ruiz-Perez, L.M.; Gonzalez-Pacanowska, D.
    Characterization of deoxyuridine 5'-triphosphate nucleotidohydrolase from Trypanosoma cruzi (2002), FEBS Lett., 526, 147-150.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.6.1.23 expressed in Escherichia coli strain BW286 Trypanosoma cruzi

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.6.1.23 5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine
-
Trypanosoma cruzi
3.6.1.23 alpha,beta-imido-dUDP
-
Trypanosoma cruzi

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.6.1.23 0.0005
-
dUTP 25°C Trypanosoma cruzi
3.6.1.23 0.0062
-
dUDP 25°C Trypanosoma cruzi

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.6.1.23 Mg2+ required Trypanosoma cruzi

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.6.1.23 32000
-
2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence Trypanosoma cruzi
3.6.1.23 32065
-
2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence Trypanosoma cruzi
3.6.1.23 62440
-
gel filtration Trypanosoma cruzi
3.6.1.23 64000
-
SDS-PAGE of cross-linked subunits Trypanosoma cruzi

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.6.1.23 dUTP + H2O Trypanosoma cruzi removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA dUMP + diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
3.6.1.23 Trypanosoma cruzi
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.6.1.23 recombinant protein Trypanosoma cruzi

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.6.1.23 dUDP + H2O
-
Trypanosoma cruzi dUMP + phosphate
-
?
3.6.1.23 dUTP + H2O
-
Trypanosoma cruzi dUMP + diphosphate
-
?
3.6.1.23 dUTP + H2O removes dUTP from the nucleotide triphosphate pool and therefore prevents the incorporation of uracil into the DNA Trypanosoma cruzi dUMP + diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
3.6.1.23 dimer 2 * 32000, SDS-PAGE, 2 * 32065, calculated from amino acid sequence Trypanosoma cruzi

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.6.1.23 2.8
-
dUTP 25°C Trypanosoma cruzi

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.6.1.23 0.00024
-
alpha,beta-imido-dUDP 25°C Trypanosoma cruzi
3.6.1.23 2.5
-
5'-O-(4,4'-dimethoxytrityl)-2'-deoxyuridine 25°C Trypanosoma cruzi

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.6.1.23 Trypanosoma cruzi calculated from amino acid sequence
-
4.9