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Literature summary extracted from

  • Koshiishi, C.; Kato, A.; Yama, S.; Crozier, A.; Ashihara, H.
    A new caffeine biosynthetic pathway in tea leaves: utilisation of adenosine released from the S-adenosyl-L-methionine cycle (2001), FEBS Lett., 499, 50-54.
    View publication on PubMed

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.1.1.159 chloroplast
-
 Camellia sinensis 9507
-
2.1.1.160 chloroplast
-
 Camellia sinensis 9507
-
3.2.2.7 chloroplast
-
 Camellia sinensis 9507
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine Camellia sinensis the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine Camellia sinensis the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine Camellia sinensis the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
3.2.2.7 additional information Camellia sinensis proposed pathway for biosynthesis of purine alkaloids with adenosine derived from SAM cycle ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.158 Camellia sinensis
-
-
-
2.1.1.159 Camellia sinensis
-
-
-
2.1.1.160 Camellia sinensis
-
-
-
3.2.2.7 Camellia sinensis
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.1.1.158 leaf activity profile of the enzyme in young, adult, and aged leaves, high activity in young leaves Camellia sinensis
-
2.1.1.159 leaf activity profile of the enzyme in young, adult, and aged leaves, high activity in young leaves Camellia sinensis
-
2.1.1.160 leaf activity profile of the enzyme in young, adult, and aged leaves, high activity in young leaves Camellia sinensis
-
3.2.2.7 leaf activity decreases rapidly during aging of leaf Camellia sinensis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.1.1.158 additional information
-
 in vivo activities after feeding of radiolabeled L-methionine, activity profile of the enzyme in young, adult, and aged leaves, overview Camellia sinensis
2.1.1.159 additional information
-
 in vivo activities after feeding of radiolabeled L-methionine, activity profile of the enzyme in young, adult, and aged leaves, overview Camellia sinensis
2.1.1.160 additional information
-
 in vivo activities after feeding of radiolabeled L-methionine, activity profile of the enzyme in young, adult, and aged leaves, overview Camellia sinensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.158 S-adenosyl-L-methionine + xanthosine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
2.1.1.158 S-adenosyl-L-methionine + xanthosine the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview Camellia sinensis S-adenosyl-L-homocysteine + 7-methylxanthosine
-
 ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.159 S-adenosyl-L-methionine + 7-methylxanthine the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview Camellia sinensis S-adenosyl-L-homocysteine + 3,7-dimethylxanthine i.e. theobromine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine
-
 Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine i.e. caffeine ?
2.1.1.160 S-adenosyl-L-methionine + 3,7-dimethylxanthine the enzyme is part of a caffeine biosynthetic pathway which includes a recycling of adenosine released from S-adenosyl-L-methionine in form of xanthosine monophosphate, overview Camellia sinensis S-adenosyl-L-homocysteine + 1,3,7-trimethylxanthine
-
 ?
3.2.2.7 additional information proposed pathway for biosynthesis of purine alkaloids with adenosine derived from SAM cycle Camellia sinensis ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.158 7-methylxanthine 3-N-methyltransferase
-
 Camellia sinensis
2.1.1.158 SAM:xanthosine 7-N-methyltransferase
-
 Camellia sinensis
2.1.1.159 7-methylxanthine 3-N-methyltransferase
-
 Camellia sinensis
2.1.1.160 CS
-
 Camellia sinensis
2.1.1.160 theobromine 1-N-methyltransferase
-
 Camellia sinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.158 27
-
 assay at Camellia sinensis
2.1.1.159 27
-
 assay at Camellia sinensis
2.1.1.160 27
-
 assay at Camellia sinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.1.1.158 8.3
-
 assay at Camellia sinensis
2.1.1.159 8.3
-
 assay at Camellia sinensis
2.1.1.160 8.3
-
 assay at Camellia sinensis