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Literature summary extracted from

  • Sorrentino, S.; Barone, R.; Bucci, E.; Gotte, G.; Russo, N.; Libonati, M.; D'Alessio, G.
    The two dimeric forms of RNase A (2000), FEBS Lett., 466, 35-39.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.6.1.18 cytosolic ribonuclease inhibitor with CpA as substrate, both isoenzymes are fully susceptible to inhibition Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
4.6.1.18 Bos taurus
-
 bovine
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.6.1.18 gel filtration and cation exchange chromatography Bos taurus

Reaction

EC Number Reaction Comment Organism Reaction ID
4.6.1.18 an [RNA] containing cytidine + H2O = an [RNA]-3'-cytidine-3'-phosphate + a 5'-hydroxy-ribonucleotide-3'-[RNA] two isoforms of dimeric enzyme, D-I and D-II Bos taurus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.6.1.18 pancreas
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.6.1.18 additional information
-
 isoenzyme D-I shows values of 88 U/mg, 382 U/mg and 6.2 U/mg with yeast RNA, poly(U) and poly(A)poly(U) as substrates, respectively. D-II isoenzyme shows values of 85 U/mg, 387 U/mg and 2.5 U/mg with yeast RNA, poly(U) and poly(A)poly(U) as substrates, respectively Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.6.1.18 cytidinyl-3',5'-adenosine + H2O CpA Bos taurus adenosine + 3'-CMP
-
 ?
4.6.1.18 poly(A)poly(U) + H2O
-
 Bos taurus ?
-
 ?
4.6.1.18 poly(U) + H2O
-
 Bos taurus 3'-UMP + 3'-oligonucleotides
-
 ?
4.6.1.18 RNA + H2O yeast RNA Bos taurus 3'-phosphomononucleotides + 3'-phosphooligonucleotides
-
 ?

Subunits

EC Number Subunits Comment Organism
4.6.1.18 dimer gel filtration Bos taurus