Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Akashi, T.; Aoki, T.; Ayabe, S.
    Identification of a cytochrome P450 cDNA encoding (2S)-flavanone 2-hydroxylase of licorice (Glycyrrhiza echinata L.; Fabaceae) which represents licodione synthase and flavone synthase II (1998), FEBS Lett., 431, 287-290.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.14.162 CYP Ge-5/CYP93B1, expression in Sf9 insect cells and in Saccharomyces cerevisiae BJ2168 Glycyrrhiza echinata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.14.162 microsome recombinant enzyme, expressed in Sf9 insect cells or yeast cells Glycyrrhiza echinata
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.14.162 additional information Glycyrrhiza echinata flavone biosynthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.14.162 Glycyrrhiza echinata P93149
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
1.14.14.162 a flavanone + [reduced NADPH-hemoprotein reductase] + O2 = a 2-hydroxyflavanone + [oxidized NADPH-hemoprotein reductase] + H2O mechanism, stereoselectivity Glycyrrhiza echinata
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.14.162 (2S)-liquiritigenin + [reduced NADPH-hemoprotein reductase] + O2 hydroxylation of liquiritigenin at C-2 and formation of licodione as a result of non-enzymatic hemiacetal opening, selectivity towards (2S)-flavanone Glycyrrhiza echinata licodione + [oxidized NADPH-hemoprotein reductase] + H2O
-
 ?
1.14.14.162 (2S)-naringenin + [reduced NADPH-hemoprotein reductase] + O2 selectivity towards (2S)-flavanone Glycyrrhiza echinata 2-hydroxynaringenin + [oxidized NADPH-hemoprotein reductase] + H2O the product is further converted into apigenin when treated with HCl ?
1.14.14.162 eriodictyol + [reduced NADPH-hemoprotein reductase] + O2
-
 Glycyrrhiza echinata luteolin + [oxidized NADPH-hemoprotein reductase] + H2O product is formed after acid treatment ?
1.14.14.162 additional information flavone biosynthesis Glycyrrhiza echinata ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.14.162 (2S)-flavanone 2-hydroxylase
-
 Glycyrrhiza echinata
1.14.14.162 F2H
-
 Glycyrrhiza echinata
1.14.14.162 LS
-
 Glycyrrhiza echinata
1.14.14.162 More CYP93B1 encodes (2S)-flavanone 2-hydroxylase, which has previously been designated to licodione synthase and flavone synthase II Glycyrrhiza echinata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.14.162 25
-
 assay at Glycyrrhiza echinata

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.14.162 cytochrome P450
-
 Glycyrrhiza echinata