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Literature summary extracted from

  • Kazuta, Y.; Tokunaga, E.; Aramaki, E.; Kondo, H.
    Identification of lysine-238 of Escherichia coli biotin carboxylase as an ATP-binding residue (1998), FEBS Lett., 427, 377-380.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
6.3.4.14 overexpression in Escherichia coli JM109 Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.14 K238A ATP-binding residue, mutant with much decreased activity, kinetic data Escherichia coli
6.3.4.14 K238Q ATP-binding residue, mutant with much decreased activity, kinetic data Escherichia coli
6.3.4.14 K238R ATP-binding residue, mutant with much decreased activity, kinetic data Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.4.14 adenosine diphosphopyridoxal ATP, ADP, inorganic phosphate and bicarbonate protect against inhibition Escherichia coli
6.3.4.14 additional information not inhibited by PLP Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.14 0.17
-
 ATP wild-type BC Escherichia coli
6.3.4.14 1.8
-
 HCO3- wild-type BC Escherichia coli
6.3.4.14 3.7
-
 HCO3- K238A mutant Escherichia coli
6.3.4.14 3.9
-
 HCO3- K238Q mutant Escherichia coli
6.3.4.14 6.8
-
 HCO3- K238R mutant Escherichia coli
6.3.4.14 8.6
-
 ATP K238Q mutant Escherichia coli
6.3.4.14 13
-
 ATP K238R mutant Escherichia coli
6.3.4.14 21
-
 ATP K238A mutant Escherichia coli
6.3.4.14 140
-
 biotin K238Q mutant Escherichia coli
6.3.4.14 160
-
 biotin wild-type BC Escherichia coli
6.3.4.14 180
-
 biotin K238A and K238R mutant Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
6.3.4.14 51000
-
 x * 51000, SDS-PAGE Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.4.14 additional information Escherichia coli fatty acid synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.14 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.14 wild-type and mutant, overexpressed BC Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
6.3.4.14 additional information
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.14 ATP + biotin + HCO3-
-
 Escherichia coli ADP + phosphate + carboxybiotin
-
 ?
6.3.4.14 ATP + biotin-carboxyl-carrier protein + HCO3- Lys-238 interacts with the gamma-phosphate group of ATP but is not involved in catalysis, BC is a subunit of acetyl-CoA carboxylase and mediates the carboxylation of enzyme-bound biotin Escherichia coli ADP + phosphate + carboxybiotin-carboxyl-carrier protein
-
 ?
6.3.4.14 additional information fatty acid synthesis Escherichia coli ?
-
 ?

Subunits

EC Number Subunits Comment Organism
6.3.4.14 ? x * 51000, SDS-PAGE Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
6.3.4.14 ATP Lys-238 is an ATP-binding residue and interacts with the gamma-phosphate group of ATP, electrostatic interaction Escherichia coli