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Literature summary extracted from

  • Hedderich, R.; Thauer, R.K.
    Methanobacterium thermoautotrophicum contains a soluble enzyme system that specifically catalyzes the reduction of the heterodisulfide of coenzyme M and 7-mercaptoheptanoylthreonine phosphate with H2 (1988), FEBS Lett., 234, 223-227.
No PubMed abstract available

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.98.1 additional information
-
 additional information Km-value for N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine is below 0.1 mM Methanothermobacter thermautotrophicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.98.1 soluble
-
 Methanothermobacter thermautotrophicus
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.98.1 Methanothermobacter thermautotrophicus
-
-
-
1.8.98.1 Methanothermobacter thermautotrophicus Marburg / DSM 2133
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.8.98.1
-
 Methanothermobacter thermautotrophicus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.8.98.1 0.2
-
-
 Methanothermobacter thermautotrophicus

Storage Stability

EC Number Storage Stability Organism
1.8.98.1 4°C, under N2, partially purified enzyme, 50 mM Tris-HCl, pH 7.6, 0.5 M NaCl, loss of 20% activity within 24 h Methanothermobacter thermautotrophicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2
-
 Methanothermobacter thermautotrophicus coenzyme B + coenzyme M
-
 ?
1.8.98.1 N-(7-[(2-sulfoethyl)dithio]heptanoyl)-3-O-phospho-L-threonine + H2
-
 Methanothermobacter thermautotrophicus Marburg / DSM 2133 coenzyme B + coenzyme M
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.8.98.1 80
-
 rapid inactivation above Methanothermobacter thermautotrophicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.8.98.1 6.3
-
 Pipes/KOH buffer Methanothermobacter thermautotrophicus
1.8.98.1 7.6
-
 20°C, Tris-HCl buffer Methanothermobacter thermautotrophicus