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Literature summary extracted from

  • Raasch, C.; Streit, W.; Schanzer, J.; Bibel, M.; Gosslar, U.; Liebl, W.
    Thermotoga maritima AglA, an extremely thermostable NAD+-, Mn2+-, and thiol-dependent alpha-glucosidase (2000), Extremophiles, 4, 189-200.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.20 2-mercaptoethanol slight activation, 600 mM are required for maximal activity, cannot substitute for DTT Thermotoga maritima
3.2.1.20 DTT dependent on reducing agents, 50 mM or more required for maximal activity, due to existence of essential sulfhydryl groups in the enzyme Thermotoga maritima
3.2.1.20 L-cysteine can only partially substitute for DTT with 3fold less activation at 60 mM Thermotoga maritima
3.2.1.20 additional information no activation by NADP+, NADH, NADPH, biotin, folic acid, riboflavin, FMN, FAD, ATP, and thiamine diphosphate Thermotoga maritima
3.2.1.20 NAD+ dependent on, 0.9 mM required Thermotoga maritima

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.20 DNA sequence determination and analysis, genomic organization of the gene cluster, functional overexpression in Escherichia coli JM83 Thermotoga maritima

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.20 Cu2+
-
 Thermotoga maritima
3.2.1.20 EDTA complete inhibition at 2 mM Thermotoga maritima
3.2.1.20 Hg2+ 98% inhibition at 0.1 mM, due to existence of essential sulfhydryl groups in the enzyme Thermotoga maritima

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.20 0.23
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 60°C Thermotoga maritima
3.2.1.20 0.53
-
 4-nitrophenyl alpha-D-galactopyranoside pH 7.0, 60°C Thermotoga maritima

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.2.1.20 Co2+ activates, can partially substitute for Mn2+ Thermotoga maritima
3.2.1.20 Mn2+ activates, dependent on, 1 mM required Thermotoga maritima
3.2.1.20 additional information Fe2+ interferes with the assay, not affected by Mg2+, Ca2+, Sr2+, Ba2+, Zn2+ Thermotoga maritima
3.2.1.20 Ni2+ activates, can partially substitute for Mn2+ Thermotoga maritima

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.20 58000
-
 2 * 58000, SDS-PAGE Thermotoga maritima
3.2.1.20 110000
-
 gel filtration Thermotoga maritima

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.20 Thermotoga maritima O33830 enzyme AglA, gene aglA
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.2.1.20 recombinant enzyme from Escherichia coli, by heat denaturation and ion exchange chromatography, large scale, to near homogeneity Thermotoga maritima

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.20 4-methylumbelliferyl alpha-D-galactopyranoside + H2O
-
 Thermotoga maritima 4-methylumbelliferone + alpha-D-galactopyranose
-
 ?
3.2.1.20 4-methylumbelliferyl alpha-D-glucopyranoside + H2O
-
 Thermotoga maritima 4-methylumbelliferone + alpha-D-glucopyranose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-galactopyranoside + H2O
-
 Thermotoga maritima 4-nitrophenol + alpha-D-galactopyranose
-
 ?
3.2.1.20 4-nitrophenyl alpha-D-glucopyranoside + H2O
-
 Thermotoga maritima 4-nitrophenol + alpha-D-glucopyranose
-
 ?
3.2.1.20 additional information substrate specificity, bulky substitutents on the aglycon side are no hindrance for hydrolysis of the glycosidic bond, no activity with cyclic maltooligosaccharides, such as alpha-, beta-, or gamma-cyclodextrin Thermotoga maritima ?
-
 ?
3.2.1.20 n-dodecyl-beta-D-maltoside + H2O
-
 Thermotoga maritima ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.20 dimer 2 * 58000, SDS-PAGE Thermotoga maritima

Synonyms

EC Number Synonyms Comment Organism
3.2.1.20 AglA
-
 Thermotoga maritima

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.20 90
-
-
 Thermotoga maritima

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
3.2.1.20 65 100 50% of maximal activity at 65°C and 100°C Thermotoga maritima

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.20 additional information
-
 enzyme is extremely thermostable Thermotoga maritima
3.2.1.20 50
-
 loss of 50% activity in 48 h Thermotoga maritima
3.2.1.20 70
-
 loss of 80% activity in 48 h Thermotoga maritima

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.20 9.12
-
 4-nitrophenyl alpha-D-glucopyranoside pH 7.0, 60°C Thermotoga maritima
3.2.1.20 10.48
-
 4-nitrophenyl alpha-D-galactopyranoside pH 7.0, 60°C Thermotoga maritima

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.20 7.5
-
-
 Thermotoga maritima

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.20 6.5 8.5 50% of maximal activity at pH 6.5 and pH 8.5 Thermotoga maritima