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Literature summary extracted from

  • Bando, M.; Inoue, T.; Oka, M.; Nakamura, K.; Kawai, K.; Obazawa, H.; Kobayashi, S.; Takehana, M.
    Isolation of ascorbate free radical reductase from rabbit lens soluble fraction (2004), Exp. Eye Res., 79, 869-873.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.5.4 0.0006
-
 NADH with substrate monodehydroascorbate Oryctolagus cuniculus
1.6.5.4 0.0012
-
 NADH with ferricyanide as substrate Oryctolagus cuniculus
1.6.5.4 0.0013
-
 ferricyanide
-
 Oryctolagus cuniculus
1.6.5.4 0.0134
-
 monodehydroascorbate
-
 Oryctolagus cuniculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.6.5.4 soluble
-
 Oryctolagus cuniculus
-
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.6.5.4 31000
-
 x * 31000, SDS-PAGE Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.6.5.4 NADH + monodehydroascorbate Oryctolagus cuniculus
-
 NAD+ + ascorbate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.6.5.4 Oryctolagus cuniculus
-
 several enzyme forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.5.4 several native enzyme forms from lens soluble fraction by gel filtration, affinity chromatography, native isoelectric focusing, and 2D-electrophoresis, about 1400fold Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.5.4 lens
-
 Oryctolagus cuniculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.5.4 2 ferricyanide + NADH enzyme also shows diaphorase activity, i.e. ferricyanide reductase Oryctolagus cuniculus 2 ferrocyanide + NAD+ + H+
-
 ?
1.6.5.4 ferricyanide + reduced 2,6-dichlorophenolindophenol enzyme also shows diaphorase activity, i.e. ferricyanide reductase Oryctolagus cuniculus ferrocyanide + 2,6-dichlorophenolindophenol
-
 ?
1.6.5.4 monodehydroascorbate + NADH
-
 Oryctolagus cuniculus ascorbate + NAD+
-
 ?
1.6.5.4 NADH + monodehydroascorbate
-
 Oryctolagus cuniculus NAD+ + ascorbate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.5.4 ? x * 31000, SDS-PAGE Oryctolagus cuniculus

Synonyms

EC Number Synonyms Comment Organism
1.6.5.4 AFR reductase
-
 Oryctolagus cuniculus
1.6.5.4 ascorbate free radical reductase
-
 Oryctolagus cuniculus

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.5.4 NADH
-
 Oryctolagus cuniculus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.6.5.4 Oryctolagus cuniculus major enzyme form, native isoelectric focusing
-
 6.9
1.6.5.4 Oryctolagus cuniculus minor enzyme forms, native isoelectric focusing
-
 7